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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 354–359

Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Koyo Watanabe, Koji Matsuura, Fukukazu Kawata, Kotaro Nagata, Jun Ning, and Hiroshi Kano  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 2, pp. 354-359 (2012)
http://dx.doi.org/10.1364/BOE.3.000354


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Abstract

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.

© 2012 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(240.6680) Optics at surfaces : Surface plasmons
(180.4243) Microscopy : Near-field microscopy

ToC Category:
Cell Studies

History
Original Manuscript: November 29, 2011
Revised Manuscript: January 18, 2012
Manuscript Accepted: January 20, 2012
Published: January 23, 2012

Citation
Koyo Watanabe, Koji Matsuura, Fukukazu Kawata, Kotaro Nagata, Jun Ning, and Hiroshi Kano, "Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites," Biomed. Opt. Express 3, 354-359 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-2-354


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