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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 360–365

Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy

Shuoqi Ye, Ran Yang, Jingwei Xiong, K. Kirk Shung, Qifa Zhou, Changhui Li, and Qiushi Ren  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 2, pp. 360-365 (2012)
http://dx.doi.org/10.1364/BOE.3.000360


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Abstract

Zebrafish play an important role in biological and biomedical research. Traditional in vivo imaging methods for studying zebrafish larvae primarily require fluorescence labeling. In this work, relying on tissue intrinsic optical absorption contrast, we acquired high resolution label-free 3D images of zebrafish larvae by using photoacoustic microscopy (PAM) in vivo. The spatial resolution reaches several microns, allowing the study of microstructures in various living organs. We demonstrated that our method has the potential to be a powerful non-invasive imaging method for studying various small animal models, including zebrafish larvae, Caenorhabditis elegans, frogs and drosophila larvae.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Photoacoustic Imaging and Spectroscopy

History
Original Manuscript: December 23, 2011
Revised Manuscript: January 19, 2012
Manuscript Accepted: January 23, 2012
Published: January 24, 2012

Citation
Shuoqi Ye, Ran Yang, Jingwei Xiong, K. Kirk Shung, Qifa Zhou, Changhui Li, and Qiushi Ren, "Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy," Biomed. Opt. Express 3, 360-365 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-2-360


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