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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 2 — Feb. 1, 2012
  • pp: 366–379

Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography

Loretta Scolaro, Robert A. McLaughlin, Blake R. Klyen, Benjamin A. Wood, Peter D. Robbins, Christobel M. Saunders, Steven L. Jacques, and David D. Sampson  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 2, pp. 366-379 (2012)

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We report the use of optical coherence tomography (OCT) to determine spatially localized optical attenuation coefficients of human axillary lymph nodes and their use to generate parametric images of lymphoid tissue. 3D-OCT images were obtained from excised lymph nodes and optical attenuation coefficients were extracted assuming a single scattering model of OCT. We present the measured attenuation coefficients for several tissue regions in benign and reactive lymph nodes, as identified by histopathology. We show parametric images of the measured attenuation coefficients as well as segmented images of tissue type based on thresholding of the attenuation coefficient values. Comparison to histology demonstrates the enhancement of contrast in parametric images relative to OCT images. This enhancement is a step towards the use of OCT for in situ assessment of lymph nodes.

© 2012 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

Original Manuscript: November 18, 2011
Revised Manuscript: January 9, 2012
Manuscript Accepted: January 18, 2012
Published: January 27, 2012

Loretta Scolaro, Robert A. McLaughlin, Blake R. Klyen, Benjamin A. Wood, Peter D. Robbins, Christobel M. Saunders, Steven L. Jacques, and David D. Sampson, "Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography," Biomed. Opt. Express 3, 366-379 (2012)

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