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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 3 — Mar. 1, 2012
  • pp: 435–446

Mechanisms of nanoparticle-mediated photomechanical cell damage

Sara Peeters, Michael Kitz, Stefan Preisser, Antoinette Wetterwald, Barbara Rothen-Rutishauser, George N. Thalmann, Christina Brandenberger, Arthur Bailey, and Martin Frenz  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 3, pp. 435-446 (2012)

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Laser-assisted killing of gold nanoparticle targeted macrophages was investigated. Using pressure transient detection, flash photography and transmission electron microscopy (TEM) imaging, we studied the mechanism of single cell damage by vapor bubble formation around gold nanospheres induced by nanosecond laser pulses. The influence of the number of irradiating laser pulses and of particle size and concentration on the threshold for acute cell damage was determined. While the single pulse damage threshold is independent of the particle size, the threshold decreases with increasing particle size when using trains of pulses. The dependence of the cell damage threshold on the nanoparticle concentration during incubation reveals that particle accumulation and distribution inside the cell plays a key role in tissue imaging or cell damaging.

© 2012 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.5180) Medical optics and biotechnology : Photodynamic therapy

ToC Category:
Cell Studies

Original Manuscript: November 28, 2011
Revised Manuscript: January 30, 2012
Manuscript Accepted: January 31, 2012
Published: February 7, 2012

Sara Peeters, Michael Kitz, Stefan Preisser, Antoinette Wetterwald, Barbara Rothen-Rutishauser, George N. Thalmann, Christina Brandenberger, Arthur Bailey, and Martin Frenz, "Mechanisms of nanoparticle-mediated photomechanical cell damage," Biomed. Opt. Express 3, 435-446 (2012)

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