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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 3 — Mar. 1, 2012
  • pp: 488–502

A handheld laser scanning confocal reflectance imaging–confocal Raman microspectroscopy system

Chetan A. Patil, Christopher L. Arrasmith, Mark A. Mackanos, David L. Dickensheets, and Anita Mahadevan-Jansen  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 3, pp. 488-502 (2012)

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Confocal reflectance microscopy and confocal Raman spectroscopy have shown potential for non-destructive analysis of samples at micron-scale resolutions. Current studies utilizing these techniques often employ large bench-top microscopes, and are not suited for use outside of laboratory settings. We have developed a microscope which combines laser scanning confocal reflectance imaging and confocal Raman spectroscopy into a compact handheld probe that is capable of high-resolution imaging and spectroscopy in a variety of settings. The compact size of the probe is largely due to the use of a MEMS mirror for beam scanning. The probe is capable of axial resolutions of up to 4 μm for the confocal imaging channel and 10 μm for the confocal Raman spectroscopy channel. Here, we report instrument design, characterize optical performance, and provide images and spectra from normal skin to demonstrate the instrument’s capabilities for clinical diagnostics.

© 2012 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.1870) Medical optics and biotechnology : Dermatology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Clinical Instrumentation

Original Manuscript: October 27, 2011
Revised Manuscript: January 13, 2012
Manuscript Accepted: January 14, 2012
Published: February 9, 2012

Chetan A. Patil, Christopher L. Arrasmith, Mark A. Mackanos, David L. Dickensheets, and Anita Mahadevan-Jansen, "A handheld laser scanning confocal reflectance imaging–confocal Raman microspectroscopy system," Biomed. Opt. Express 3, 488-502 (2012)

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