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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 4 — Apr. 1, 2012
  • pp: 715–734

Adaptive optics retinal imaging in the living mouse eye

Ying Geng, Alfredo Dubra, Lu Yin, William H. Merigan, Robin Sharma, Richard T. Libby, and David R. Williams  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 4, pp. 715-734 (2012)

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Correction of the eye’s monochromatic aberrations using adaptive optics (AO) can improve the resolution of in vivo mouse retinal images [Biss et al., Opt. Lett. 32(6), 659 (2007) and Alt et al., Proc. SPIE 7550, 755019 (2010)], but previous attempts have been limited by poor spot quality in the Shack-Hartmann wavefront sensor (SHWS). Recent advances in mouse eye wavefront sensing using an adjustable focus beacon with an annular beam profile have improved the wavefront sensor spot quality [Geng et al., Biomed. Opt. Express 2(4), 717 (2011)], and we have incorporated them into a fluorescence adaptive optics scanning laser ophthalmoscope (AOSLO). The performance of the instrument was tested on the living mouse eye, and images of multiple retinal structures, including the photoreceptor mosaic, nerve fiber bundles, fine capillaries and fluorescently labeled ganglion cells were obtained. The in vivo transverse and axial resolutions of the fluorescence channel of the AOSLO were estimated from the full width half maximum (FWHM) of the line and point spread functions (LSF and PSF), and were found to be better than 0.79 μm ± 0.03 μm (STD)(45% wider than the diffraction limit) and 10.8 μm ± 0.7 μm (STD)(two times the diffraction limit), respectively. The axial positional accuracy was estimated to be 0.36 μm. This resolution and positional accuracy has allowed us to classify many ganglion cell types, such as bistratified ganglion cells, in vivo.

© 2012 OSA

OCIS Codes
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(330.7324) Vision, color, and visual optics : Visual optics, comparative animal models
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Ophthalmology Applications

Original Manuscript: January 3, 2012
Revised Manuscript: February 13, 2012
Manuscript Accepted: February 14, 2012
Published: March 15, 2012

Ying Geng, Alfredo Dubra, Lu Yin, William H. Merigan, Robin Sharma, Richard T. Libby, and David R. Williams, "Adaptive optics retinal imaging in the living mouse eye," Biomed. Opt. Express 3, 715-734 (2012)

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