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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 4 — Apr. 1, 2012
  • pp: 753–763

Mechanochemistry of single red blood cells monitored using Raman tweezers

Saurabh Raj, Mónica Marro, Michal Wojdyla, and Dmitri Petrov  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 4, pp. 753-763 (2012)
http://dx.doi.org/10.1364/BOE.3.000753


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Abstract

Two microparticles were biochemically attached to a red blood cell at diametrically opposite parts and held by optical traps allowing to impose deformations. The cell deformation was monitored from the microscopy images. Raman spectra of the cell under tunable deformations were studied. Vibrational spectra analysis at different stretching states was supported with two statistical methods. Principal Component Analysis distinguishes the most prominent changes in spectra while 2D correlation technique monitors the evolution of Raman bands during stretching. The measurements show significant changes in the cell chemical structure with stretching however the changes saturate above 20% of cell deformation. Mechanical deformation of the cell mainly affects the bands corresponding to hemoglobin but contributions from spectrin and membrane proteins can not be excluded. The saturation of bands at higher deformations suggests some structural relaxation that RBC has to undergo to bear extra load. The results confirm widely accepted belief that spectrin released from membrane proteins allows for significant shape changes of the cells. We therefore tentatively suggest that interaction between membrane and cytoskeleton during deformation can be efficiently probed by confocal Raman spectroscopy, in particular via the peak around 1035 cm−1.

© 2012 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Cell Studies

History
Original Manuscript: January 24, 2012
Revised Manuscript: March 13, 2012
Manuscript Accepted: March 13, 2012
Published: March 22, 2012

Citation
Saurabh Raj, Mónica Marro, Michal Wojdyla, and Dmitri Petrov, "Mechanochemistry of single red blood cells monitored using Raman tweezers," Biomed. Opt. Express 3, 753-763 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-4-753


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