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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 5 — May. 1, 2012
  • pp: 840–853

Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration

Donald A. Peyrot, Claire Lefort, Marie Steffenhagen, Tigran Mansuryan, Guillaume Ducourthial, Darine Abi-Haidar, Nicolas Sandeau, Christine Vever-Bizet, Sergei G. Kruglik, Luc Thiberville, Frédéric Louradour, and Geneviève Bourg-Heckly  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 5, pp. 840-853 (2012)

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Several major lung pathologies are characterized by early modifications of the extracellular matrix (ECM) fibrillar collagen and elastin network. We report here the development of a nonlinear fiber-optic spectrometer, compatible with an endoscopic use, primarily intended for the recording of second-harmonic generation (SHG) signal of collagen and two-photon excited fluorescence (2PEF) of both collagen and elastin. Fiber dispersion is accurately compensated by the use of a specific grism-pair stretcher, allowing laser pulse temporal width around 70 fs and excitation wavelength tunability from 790 to 900 nm. This spectrometer was used to investigate the excitation wavelength dependence (from 800 to 870 nm) of SHG and 2PEF spectra originating from ex vivo human lung tissue samples. The results were compared with spectral responses of collagen gel and elastin powder reference samples and also with data obtained using standard nonlinear microspectroscopy. The excitation-wavelength-tunable nonlinear fiber-optic spectrometer presented in this study allows performing nonlinear spectroscopy of human lung tissue ECM through the elastin 2PEF and the collagen SHG signals. This work opens the way to tunable excitation nonlinear endomicroscopy based on both distal scanning of a single optical fiber and proximal scanning of a fiber-optic bundle.

© 2012 OSA

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(190.4180) Nonlinear optics : Multiphoton processes
(300.6420) Spectroscopy : Spectroscopy, nonlinear

ToC Category:
Endoscopes, Catheters and Micro-Optics

Original Manuscript: March 2, 2012
Revised Manuscript: March 23, 2012
Manuscript Accepted: March 23, 2012
Published: April 3, 2012

Donald A. Peyrot, Claire Lefort, Marie Steffenhagen, Tigran Mansuryan, Guillaume Ducourthial, Darine Abi-Haidar, Nicolas Sandeau, Christine Vever-Bizet, Sergei G. Kruglik, Luc Thiberville, Frédéric Louradour, and Geneviève Bourg-Heckly, "Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration," Biomed. Opt. Express 3, 840-853 (2012)

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