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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 5 — May. 1, 2012
  • pp: 854–862

Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope

Jesse W. Wilson, Prathyush Samineni, Warren S. Warren, and Martin C. Fischer  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 5, pp. 854-862 (2012)

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Microscopy with nonlinear phase contrast is achieved by a simple modification to a nonlinear pump-probe microscope. The technique measures cross-phase modulation by detecting a pump-induced spectral shift in the probe pulse. Images with nonlinear phase contrast are acquired both in transparent and absorptive media. In paraffin-embedded biopsy sections, cross-phase modulation complements the chemically-specific pump-probe images with structural context.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: March 9, 2012
Revised Manuscript: April 1, 2012
Manuscript Accepted: April 3, 2012
Published: April 4, 2012

Jesse W. Wilson, Prathyush Samineni, Warren S. Warren, and Martin C. Fischer, "Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope," Biomed. Opt. Express 3, 854-862 (2012)

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  1. D. Fu, T. E. Matthews, T. Ye, I. R. Piletic, and W. S. Warren, “Label-free in vivo optical imaging of microvasculature and oxygenation level,” J. Biomed. Opt.13(4), 040503 (2008). [CrossRef] [PubMed]
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