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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 5 — May. 1, 2012
  • pp: 920–926

Reusable molecular sensor based on photonic activation control of DNA probes

Takahiro Nishimura, Yusuke Ogura, and Jun Tanida  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 5, pp. 920-926 (2012)

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We propose a photoactivatable and resettable molecular sensor using DNA probes. The functionality is achieved by reversible change of DNA structure induced via photonic signals. Based on the mechanism, the sensor can detect target molecules at a desired instant and can be returned to its initial state after detection. The experimental results demonstrate that the concentrations of the target molecules are detected correctly and repeatedly according to the light signal.

© 2012 OSA

OCIS Codes
(350.5130) Other areas of optics : Photochemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Biosensors and Molecular Diagnostics

Original Manuscript: March 14, 2012
Revised Manuscript: April 3, 2012
Manuscript Accepted: April 4, 2012
Published: April 11, 2012

Takahiro Nishimura, Yusuke Ogura, and Jun Tanida, "Reusable molecular sensor based on photonic activation control of DNA probes," Biomed. Opt. Express 3, 920-926 (2012)

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  1. K. Wang, Z. Tang, C. J. Yang, Y. Kim, X. Fang, W. Li, Y. Wu, C. D. Medley, Z. Cao, J. Li, P. Colon, H. Lin, and W. Tan, “Molecular engineering of DNA: molecular beacons,” Angew. Chem. Int. Ed.48, 856–870 (2009). [CrossRef]
  2. D. P. Bratu, B. J. Cha, M. M. Mhlanga, F. R. Kramer, and S. Tyagi, “Visualizing the distribution and transport of mRNAs in living cells,” Proc. Natl. Acad. Sci.100, 13308–13313 (2003). [CrossRef] [PubMed]
  3. H. Asanuma, X. Liang, H. Nishioka, D. Matsunaga, M. Liu, and M. Komiyama, “Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription,” Nat. Protocols2, 203–212 (2007). [CrossRef]
  4. H. Asanuma, T. Ito, T. Yoshida, X. Liang, and M. Komiyama, “Photo-regulation of the formation and dissociation of DNA-duplex by using the cis-trans isomerization of azobenzene,” Angew. Chem. Int. Ed.38, 2393–2395 (1999). [CrossRef]
  5. S. Ogasawara and M. Maeda, “Straightforward and reversible photoregulation of hybridization by using a photochromic nucleoside,” Angew. Chem. Int. Ed.47, 8839–8842 (2008). [CrossRef]
  6. L. Poon, W. Zandberg, D. Hsiao, Z. Erno, D. Sen, B. D. Gates, and N. R. Branda, “Photothermal release of single-stranded DNA from the surface of gold nanoparticles through controlled denaturating and Au–S bond breaking,” ACS Nano4, 6395–6403 (2010). [CrossRef] [PubMed]
  7. X. Liang, H. Nishioka, N. Takenaka, and H. Asanuma, “A DNA nanomachine powered by light irradiation,” ChemBioChem9, 702–705 (2008). [CrossRef] [PubMed]
  8. Y. Ogura, T. Nishimura, and J. Tanida, “Self-contained photonically-controlled DNA tweezers,” Appl. Phys. Express2, 025004 (2009). [CrossRef]
  9. H. Kang, H. Liu, J. A. Phillips, Z. Cao, Y. Kim, Y. Chen, Z. Yang, J. Li, and W. Tan, “Single-DNA molecule nanomotor regulated by photons,” Nano Lett.9, 2690–2696 (2009). [CrossRef] [PubMed]
  10. X. Liang, T. Mochizuki, and H. Asanuma, “A supra-photoswitch involving sandwiched DNA base pairs and azobenzenes for light-driven nanostructures and nanodevices,” Small5, 1761–1768 (2009). [CrossRef] [PubMed]
  11. L. Wu, K. Koumoto, and N. Sugimoto, “Reversible stability switching of a hairpin DNA via a photo-responsive linker unit,” Chem. Commun.47, 1915–1917 (2009). [CrossRef]
  12. Y. Kim, J. A. Phillipsa, H. Liua, H. Kanga, and W. Tan, “Using photons to manipulate enzyme inhibition by an azobenzene-modified nucleic acid probe,” Proc. Natl. Acad. Sci.106, 6489–6494 (2009). [CrossRef] [PubMed]
  13. M. Zhou, X. Liang, T. Mochizuki, and H. Asanuma, “A light-driven DNA nanomachine for the efficient photo-switching of RNA digestion,” Angew. Chem. Int. Ed.49, 2167–2170 (2010). [CrossRef]
  14. C. Wang, Z. Zhu, Y. Song, H. Lin, C. J. Yang, and W. Tan, “Caged molecular beacons: controlling nucleic acid hybridization with light,” Chem. Commun.47, 5708–5710 (2011). [CrossRef]
  15. T. Nishimura, Y. Ogura, and J. Tanida, “A photonic DNA processor: concept and implementation,” Proc. SPIE8102, 810207 (2011). [CrossRef]

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