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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1241–1255

Depth-resolved cellular microrheology using HiLo microscopy

Jarett Michaelson, Heejin Choi, Peter So, and Hayden Huang  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 6, pp. 1241-1255 (2012)
http://dx.doi.org/10.1364/BOE.3.001241


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Abstract

It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 μm or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.

© 2012 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy

ToC Category:
Cell Studies

History
Original Manuscript: March 21, 2012
Revised Manuscript: April 25, 2012
Manuscript Accepted: April 29, 2012
Published: May 3, 2012

Citation
Jarett Michaelson, Heejin Choi, Peter So, and Hayden Huang, "Depth-resolved cellular microrheology using HiLo microscopy," Biomed. Opt. Express 3, 1241-1255 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-6-1241


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