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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1291–1299

Designing microarray phantoms for hyperspectral imaging validation

Matthew L. Clarke, Ji Youn Lee, Daniel V. Samarov, David W. Allen, Maritoni Litorja, Ralph Nossal, and Jeeseong Hwang  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 6, pp. 1291-1299 (2012)

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The design and fabrication of custom-tailored microarrays for use as phantoms in the characterization of hyperspectral imaging systems is described. Corresponding analysis methods for biologically relevant samples are also discussed. An image-based phantom design was used to program a microarrayer robot to print prescribed mixtures of dyes onto microscope slides. The resulting arrays were imaged by a hyperspectral imaging microscope. The shape of the spots results in significant scattering signals, which can be used to test image analysis algorithms. Separation of the scattering signals allowed elucidation of individual dye spectra. In addition, spectral fitting of the absorbance spectra of complex dye mixtures was performed in order to determine local dye concentrations. Such microarray phantoms provide a robust testing platform for comparisons of hyperspectral imaging acquisition and analysis methods.

© 2012 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(350.4800) Other areas of optics : Optical standards and testing
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Calibration, Validation and Phantom Studies

Original Manuscript: March 2, 2012
Revised Manuscript: April 13, 2012
Manuscript Accepted: April 15, 2012
Published: May 9, 2012

Virtual Issues
Phantoms for the Performance Evaluation and Validation of Optical Medical Imaging Devices (2012) Biomedical Optics Express

Matthew L. Clarke, Ji Youn Lee, Daniel V. Samarov, David W. Allen, Maritoni Litorja, Ralph Nossal, and Jeeseong Hwang, "Designing microarray phantoms for hyperspectral imaging validation," Biomed. Opt. Express 3, 1291-1299 (2012)

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