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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1365–1380

Diabetes imaging—quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Corinne Berclaz, Joan Goulley, Martin Villiger, Christophe Pache, Arno Bouwens, Erica Martin-Williams, Dimitri Van de Ville, Anthony C. Davison, Anne Grapin-Botton, and Theo Lasser  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 6, pp. 1365-1380 (2012)

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Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Coherence Microscopy (xfOCM). To identify and quantify the islets of Langerhans observed in xfOCM tomograms we implemented an active contour algorithm based on the level set method. We show that xfOCM reveals a three-dimensional islet distribution consistent with Optical Projection Tomography, albeit with a higher resolution that also enables the detection of the smallest islets (≤ 8000 μm3). Although this category of the smallest islets represents only a negligible volume compared to the total β-cell volume, a recent study suggests that these islets, located at the periphery, are the first to be destroyed when type I diabetes develops. Our results underline the capability of xfOCM to contribute to the understanding of the development of diabetes, especially when considering islet volume distribution instead of the total β-cell volume only.

© 2012 OSA

OCIS Codes
(100.6890) Image processing : Three-dimensional image processing
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Optical Coherence Tomography

Original Manuscript: April 12, 2012
Revised Manuscript: May 7, 2012
Manuscript Accepted: May 10, 2012
Published: May 14, 2012

Corinne Berclaz, Joan Goulley, Martin Villiger, Christophe Pache, Arno Bouwens, Erica Martin-Williams, Dimitri Van de Ville, Anthony C. Davison, Anne Grapin-Botton, and Theo Lasser, "Diabetes imaging—quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy," Biomed. Opt. Express 3, 1365-1380 (2012)

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