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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1433–1445

Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds

Ronald X. Xu, David W. Allen, Jiwei Huang, Surya Gnyawali, James Melvin, Haytham Elgharably, Gayle Gordillo, Kun Huang, Valerie Bergdall, Maritoni Litorja, Joseph P. Rice, Jeeseong Hwang, and Chandan K. Sen  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 6, pp. 1433-1445 (2012)

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Hyperspectral imaging has the potential to achieve high spatial resolution and high functional sensitivity for non-invasive assessment of tissue oxygenation. However, clinical acceptance of hyperspectral imaging in ischemic wound assessment is hampered by its poor reproducibility, low accuracy, and misinterpreted biology. These limitations are partially caused by the lack of a traceable calibration standard. We proposed a digital tissue phantom (DTP) platform for quantitative calibration and performance evaluation of spectral wound imaging devices. The technical feasibility of such a DTP platform was demonstrated by both in vitro and in vivo experiments. The in vitro DTPs were developed based on a liquid blood phantom model. The in vivo DTPs were developed based on a porcine ischemic skin flap model. The DTPs were projected by a Hyperspectral Image Projector (HIP) with high fidelity. A wide-gap 2nd derivative oxygenation algorithm was developed to reconstruct tissue functional parameters from hyperspectral measurements. In this study, we have demonstrated not only the technical feasibility of using DTPs for quantitative calibration, evaluation, and optimization of spectral imaging devices but also its potential for ischemic wound assessment in clinical practice.

© 2012 OSA

OCIS Codes
(120.4800) Instrumentation, measurement, and metrology : Optical standards and testing
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

ToC Category:
Calibration, Validation and Phantom Studies

Original Manuscript: March 2, 2012
Revised Manuscript: May 3, 2012
Manuscript Accepted: May 4, 2012
Published: May 18, 2012

Virtual Issues
Phantoms for the Performance Evaluation and Validation of Optical Medical Imaging Devices (2012) Biomedical Optics Express

Ronald X. Xu, David W. Allen, Jiwei Huang, Surya Gnyawali, James Melvin, Haytham Elgharably, Gayle Gordillo, Kun Huang, Valerie Bergdall, Maritoni Litorja, Joseph P. Rice, Jeeseong Hwang, and Chandan K. Sen, "Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds," Biomed. Opt. Express 3, 1433-1445 (2012)

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