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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 6 — Jun. 1, 2012
  • pp: 1446–1454

In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy

S. Bancelin, C. Aimé, T. Coradin, and M.-C. Schanne-Klein  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 6, pp. 1446-1454 (2012)
http://dx.doi.org/10.1364/BOE.3.001446


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Abstract

We implemented in situ time-lapse Second Harmonic Generation (SHG) microscopy to monitor the three-dimensional (3D) self-assembly of collagen in solution. As a proof of concept, we tuned the kinetics of fibril formation by varying the pH and measured the subsequent exponential increase of fibril volume density in SHG images. We obtained significantly different time constants at pH = 6.5 ± 0.3 and at pH = 7.5 ± 0.3. Moreover, we showed that we could focus on the growth of a single isolated collagen fibril because SHG microscopy is sensitive to well-organized fibrils with diameter below the optical resolution. This work illustrates the potential of SHG microscopy for the rational design and characterization of collagen-based biomaterials.

© 2012 OSA

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.2620) Nonlinear optics : Harmonic generation and mixing
(190.4180) Nonlinear optics : Multiphoton processes
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Microscopy

History
Original Manuscript: April 18, 2012
Revised Manuscript: May 16, 2012
Manuscript Accepted: May 16, 2012
Published: May 21, 2012

Citation
S. Bancelin, C. Aimé, T. Coradin, and M.-C. Schanne-Klein, "In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy," Biomed. Opt. Express 3, 1446-1454 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-6-1446


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