OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 7 — Jul. 1, 2012
  • pp: 1492–1505

Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles

Omar E. Olarte, Jacob Licea-Rodriguez, Jonathan A. Palero, Emilio J. Gualda, David Artigas, Jürgen Mayer, Jim Swoger, James Sharpe, Israel Rocha-Mendoza, Raul Rangel-Rojo, and Pablo Loza-Alvarez  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 7, pp. 1492-1505 (2012)
http://dx.doi.org/10.1364/BOE.3.001492


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Abstract

We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.

© 2012 OSA

OCIS Codes
(140.3300) Lasers and laser optics : Laser beam shaping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 14, 2012
Revised Manuscript: May 22, 2012
Manuscript Accepted: May 31, 2012
Published: June 4, 2012

Citation
Omar E. Olarte, Jacob Licea-Rodriguez, Jonathan A. Palero, Emilio J. Gualda, David Artigas, Jürgen Mayer, Jim Swoger, James Sharpe, Israel Rocha-Mendoza, Raul Rangel-Rojo, and Pablo Loza-Alvarez, "Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles," Biomed. Opt. Express 3, 1492-1505 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-7-1492


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