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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 7 — Jul. 1, 2012
  • pp: 1750–1756

Multimodal microscopy with sub-30 fs Yb fiber laser oscillator

Bai Nie, Ilyas Saytashev, Andy Chong, Hui Liu, Sergey N. Arkhipov, Frank W. Wise, and Marcos Dantus  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 7, pp. 1750-1756 (2012)

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Nonlinear optical microscopy with sub-30 fs pulses from an Yb-fiber laser, approximately three times shorter than typical fiber laser pulses, leads to an order of magnitude brighter third harmonic generation imaging. Multiphoton fluorescence, second and third harmonic generation modalities are compared on stained microspheres and unstained biological tissues.

© 2012 OSA

OCIS Codes
(060.2320) Fiber optics and optical communications : Fiber optics amplifiers and oscillators
(110.2350) Imaging systems : Fiber optics imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: April 25, 2012
Revised Manuscript: June 8, 2012
Manuscript Accepted: June 9, 2012
Published: June 27, 2012

Bai Nie, Ilyas Saytashev, Andy Chong, Hui Liu, Sergey N. Arkhipov, Frank W. Wise, and Marcos Dantus, "Multimodal microscopy with sub-30 fs Yb fiber laser oscillator," Biomed. Opt. Express 3, 1750-1756 (2012)

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  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science248(4951), 73–76 (1990). [CrossRef] [PubMed]
  2. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol.21(11), 1369–1377 (2003). [CrossRef] [PubMed]
  3. K. König, “Multiphoton microscopy in life sciences,” J. Microsc.200(2), 83–104 (2000). [CrossRef] [PubMed]
  4. S. Maiti, J. B. Shear, R. M. Williams, W. R. Zipfel, and W. W. Webb, “Measuring serotonin distribution in live cells with three-photon excitation,” Science275(5299), 530–532 (1997). [CrossRef] [PubMed]
  5. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, “Nonlinear scanning laser microscopy by third harmonic generation,” Appl. Phys. Lett.70(8), 922–924 (1997). [CrossRef]
  6. J. A. Squier, M. Muller, G. J. Brakenhoff, and K. R. Wilson, “Third harmonic generation microscopy,” Opt. Express3(9), 315–324 (1998). [CrossRef] [PubMed]
  7. P. Xi, Y. Andegeko, D. Pestov, V. V. Lovozoy, and M. Dantus, “Two-photon imaging using adaptive phase compensated ultrashort laser pulses,” J. Biomed. Opt.14(1), 014002 (2009). [CrossRef] [PubMed]
  8. D. Yelin, D. Oron, E. Korkotian, M. Segal, and Y. Silberberg, “Third-harmonic microscopy with a titanium-sapphire laser,” Appl. Phys. B74(9), S97–S101 (2002). [CrossRef]
  9. C. K. Sun, S. W. Chu, S. P. Tai, S. Keller, U. K. Mishra, and S. P. DenBaars, “Scanning second-harmonic/third-harmonic generation microscopy of gallium nitride,” Appl. Phys. Lett.77(15), 2331–2333 (2000). [CrossRef]
  10. A. Chong, J. Buckley, W. Renninger, and F. Wise, “All-normal-dispersion femtosecond fiber laser,” Opt. Express14(21), 10095–10100 (2006). [CrossRef] [PubMed]
  11. W. H. Renninger, A. Chong, and F. W. Wise, “Self-similar pulse evolution in an all-normal-dispersion laser,” Phys. Rev. A82(2), 021805 (2010). [CrossRef] [PubMed]
  12. B. Nie, D. Pestov, F. W. Wise, and M. Dantus, “Generation of 42-fs and 10-nJ pulses from a fiber laser with self-similar evolution in the gain segment,” Opt. Express19(13), 12074–12080 (2011). [CrossRef] [PubMed]
  13. A. C. Millard, P. W. Wiseman, D. N. Fittinghoff, K. R. Wilson, J. A. Squier, and M. Müller, “Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source,” Appl. Opt.38(36), 7393–7397 (1999). [CrossRef] [PubMed]
  14. G. J. Liu, K. Kieu, F. W. Wise, and Z. P. Chen, “Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe,” J Biophotonics4(1-2), 34–39 (2011). [CrossRef] [PubMed]
  15. A. Besaratinia, J. I. Yoon, C. Schroeder, S. E. Bradforth, M. Cockburn, and G. P. Pfeifer, “Wavelength dependence of ultraviolet radiation-induced DNA damage as determined by laser irradiation suggests that cyclobutane pyrimidine dimers are the principal DNA lesions produced by terrestrial sunlight,” FASEB J.25(9), 3079–3091 (2011). [CrossRef] [PubMed]
  16. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express17(16), 13354–13364 (2009). [CrossRef] [PubMed]
  17. A. Chong, H. Liu, B. Nie, B. G. Bale, S. Wabnitz, W. H. Renninger, M. Dantus, and F. W. Wise, “Pulse generation without gain-bandwidth limitation in a laser with self-similar evolution,” Opt. Express20(13), 14213 –14220 (2012). [CrossRef]
  18. V. V. Lozovoy, I. Pastirk, and M. Dantus, “Multiphoton intrapulse interference. IV. Ultrashort laser pulse spectral phase characterization and compensation,” Opt. Lett.29(7), 775–777 (2004). [CrossRef] [PubMed]
  19. B. Xu, J. M. Gunn, J. M. D. Cruz, V. V. Lozovoy, and M. Dantus, “Quantitative investigation of the multiphoton intrapulse interference phase scan method for simultaneous phase measurement and compensation of femtosecond laser pulses,” J. Opt. Soc. Am. B23(4), 750–759 (2006). [CrossRef]
  20. D. Pestov, V. V. Lozovoy, and M. Dantus, “Multiple Independent Comb Shaping (MICS): phase-only generation of optical pulse sequences,” Opt. Express17(16), 14351–14361 (2009). [CrossRef] [PubMed]
  21. M. M. Wefers and K. A. Nelson, “Space-time profiles of shaped ultrafast optical waveforms,” IEEE J. Quantum Electron.32(1), 161–172 (1996). [CrossRef]
  22. F. Frei, A. Galler, and T. Feurer, “Space-time coupling in femtosecond pulse shaping and its effects on coherent control,” J. Chem. Phys.130(3), 034302 (2009). [CrossRef] [PubMed]
  23. D. Brinks, F. D. Stefani, and N. F. Hulst, “Nanoscale spatial effects of pulse shaping,” Ultrafast Phenomena XVI92, 890–892 (2009). [CrossRef]
  24. N. Krebs, R. A. Probst, and E. Riedle, “Sub-20 fs pulses shaped directly in the UV by an acousto-optic programmable dispersive filter,” Opt. Express18(6), 6164–6171 (2010). [CrossRef] [PubMed]

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