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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 8 — Aug. 1, 2012
  • pp: 1841–1854

Structured oblique illumination microscopy for enhanced resolution imaging of non-fluorescent, coherently scattering samples

Shwetadwip Chowdhury, Al-Hafeez Dhalla, and Joseph Izatt  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 8, pp. 1841-1854 (2012)

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Many biological structures of interest are beyond the diffraction limit of conventional microscopes and their visualization requires application of super-resolution techniques. Such techniques have found remarkable success in surpassing the diffraction limit to achieve sub-diffraction limited resolution; however, they are predominantly limited to fluorescent samples. Here, we introduce a non-fluorescent analogue to structured illumination microscopy, termed structured oblique illumination microscopy (SOIM), where we use simultaneous oblique illuminations of the sample to multiplex high spatial-frequency content into the frequency support of the system. We introduce a theoretical framework describing how to demodulate this multiplexed information to reconstruct an image with a spatial-frequency support exceeding that of the system’s classical diffraction limit. This approach allows enhanced-resolution imaging of non-fluorescent samples. Experimental confirmation of the approach is obtained in a reflection test target with moderate numerical aperture.

© 2012 OSA

OCIS Codes
(030.0030) Coherence and statistical optics : Coherence and statistical optics
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: April 25, 2012
Revised Manuscript: June 13, 2012
Manuscript Accepted: June 24, 2012
Published: July 12, 2012

Shwetadwip Chowdhury, Al-Hafeez Dhalla, and Joseph Izatt, "Structured oblique illumination microscopy for enhanced resolution imaging of non-fluorescent, coherently scattering samples," Biomed. Opt. Express 3, 1841-1854 (2012)

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