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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 8 — Aug. 1, 2012
  • pp: 1914–1923

Multispectral nanoparticle contrast agents for true-color spectroscopic optical coherence tomography

You Leo Li, Kevin Seekell, Hsiangkuo Yuan, Francisco E. Robles, and Adam Wax  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 8, pp. 1914-1923 (2012)

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We have recently developed a novel dual window scheme for processing spectroscopic OCT images to provide spatially resolved true color imaging of chromophores in scattering samples. Here we apply this method to measure the extinction spectra of plasmonic nanoparticles at various concentrations for potential in vivo applications. We experimentally demonstrate sub-nanomolar sensitivity in the measurement of nanoparticle concentrations, and show that colorimetric imaging with multiple species of nanoparticles produces enhanced contrast for spectroscopic OCT in both tissue phantom and cell studies.

© 2012 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(300.1030) Spectroscopy : Absorption
(160.4236) Materials : Nanomaterials

ToC Category:
Optical Coherence Tomography

Original Manuscript: June 7, 2012
Revised Manuscript: July 18, 2012
Manuscript Accepted: July 18, 2012
Published: July 20, 2012

Virtual Issues
BIOMED 2012 (2012) Biomedical Optics Express

You Leo Li, Kevin Seekell, Hsiangkuo Yuan, Francisco E. Robles, and Adam Wax, "Multispectral nanoparticle contrast agents for true-color spectroscopic optical coherence tomography," Biomed. Opt. Express 3, 1914-1923 (2012)

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  1. M. Hu, J. Chen, Z.-Y. Li, L. Au, G. V. Hartland, X. Li, M. Marquez, and Y. Xia, “Gold nanostructures: engineering their plasmonic properties for biomedical applications,” Chem. Soc. Rev.35(11), 1084–1094 (2006). [CrossRef] [PubMed]
  2. A. L. Oldenburg, M. N. Hansen, D. A. Zweifel, A. Wei, and S. A. Boppart, “Plasmon-resonant gold nanorods as low backscattering albedo contrast agents for optical coherence tomography,” Opt. Express14(15), 6724–6738 (2006). [CrossRef] [PubMed]
  3. A. L. Oldenburg, M. N. Hansen, T. S. Ralston, A. Wei, and S. A. Boppart, “Imaging gold nanorods in excised human breast carcinoma by spectroscopic optical coherence tomography,” J. Mater. Chem.19(35), 6407–6411 (2009). [CrossRef] [PubMed]
  4. F. E. Robles, S. Chowdhury, and A. Wax, “Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics,” Biomed. Opt. Express1(1), 310–317 (2010). [CrossRef] [PubMed]
  5. F. E. Robles, C. Wilson, G. Grant, and A. Wax, “Molecular imaging true-colour spectroscopic optical coherence tomography,” Nat. Photonics5(12), 744–747 (2011). [CrossRef]
  6. R. N. Graf, W. J. Brown, and A. Wax, “Parallel frequency-domain optical coherence tomography scatter-mode imaging of the hamster cheek pouch using a thermal light source,” Opt. Lett.33(12), 1285–1287 (2008). [CrossRef] [PubMed]
  7. A. Wax, C. Yang, R. R. Dasari, and M. S. Feld, “Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy,” Opt. Lett.26(6), 322–324 (2001). [CrossRef] [PubMed]
  8. F. Robles, R. N. Graf, and A. Wax, “Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution,” Opt. Express17(8), 6799–6812 (2009). [CrossRef] [PubMed]
  9. B. Duncan, C. Kim, and V. M. Rotello, “Gold nanoparticle platforms as drug and biomacromolecule delivery systems,” J. Control. Release148(1), 122–127 (2010). [CrossRef] [PubMed]
  10. A. K. Salem, P. C. Searson, and K. W. Leong, “Multifunctional nanorods for gene delivery,” Nat. Mater.2(10), 668–671 (2003). [CrossRef] [PubMed]
  11. A. P. Leonov, J. Zheng, J. D. Clogston, S. T. Stern, A. K. Patri, and A. Wei, “Detoxification of gold nanorods by treatment with polystyrenesulfonate,” ACS Nano2(12), 2481–2488 (2008). [CrossRef] [PubMed]
  12. K. Seekell, H. Price, S. Marinakos, and A. Wax, “Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis,” Methods56(2), 310–316 (2012). [CrossRef] [PubMed]
  13. A. Curatolo, B. F. Kennedy, and D. D. Sampson, “Structured three-dimensional optical phantom for optical coherence tomography,” Opt. Express19(20), 19480–19485 (2011). [CrossRef] [PubMed]
  14. M. C. Skala, M. J. Crow, A. Wax, and J. A. Izatt, “Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres,” Nano Lett.8(10), 3461–3467 (2008). [CrossRef] [PubMed]
  15. H. Yuan, A. M. Fales, and T. Vo-Dinh, “TAT peptide-functionalized gold nanostars: enhanced intracellular delivery and efficient NIR photothermal therapy using ultralow irradiance,” J. Am. Chem. Soc.134(28), 11358–11361 (2012). [PubMed]
  16. A. C. Curry, M. Crow, and A. Wax, “Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles,” J. Biomed. Opt.13(1), 014022 (2008). [CrossRef] [PubMed]
  17. K. Seekell, M. J. Crow, S. Marinakos, J. Ostrander, A. Chilkoti, and A. Wax, “Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles,” J. Biomed. Opt.16(11), 116003 (2011). [CrossRef] [PubMed]

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