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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 8 — Aug. 1, 2012
  • pp: 1924–1932

Nondestructive volumetric imaging of tissue microstructure with benchtop x-ray phase-contrast tomography and critical point drying

Adam M. Zysk, Alfred B. Garson, III, Qiaofeng Xu, Eric M. Brey, Wei Zhou, Jovan G. Brankov, Miles N. Wernick, Jerome R. Kuszak, and Mark A. Anastasio  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 8, pp. 1924-1932 (2012)

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The in vitro investigation of many optically opaque biological microstructures requires 3D analysis at high resolution over a large field of view. We demonstrate a new nondestructive volumetric imaging technique that eliminates the structural and computational limitations of conventional 2D optical microscopy by combining x-ray phase-contrast tomography with critical point drying sample preparation. We experimentally demonstrate the enhancement of small features afforded by phase-contrast imaging and show the contrast improvement afforded by the drying of a hydrated specimen. We further demonstrate the biological application of this technique by imaging the microstructure of the accommodative apparatus in a primate eye using a benchtop phase-contrast tomography system.

© 2012 OSA

OCIS Codes
(110.7440) Imaging systems : X-ray imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.6960) Medical optics and biotechnology : Tomography
(350.5030) Other areas of optics : Phase
(170.6935) Medical optics and biotechnology : Tissue characterization
(330.7322) Vision, color, and visual optics : Visual optics, accommodation

ToC Category:
X-Ray Microscopy and Imaging

Original Manuscript: May 10, 2012
Revised Manuscript: July 19, 2012
Manuscript Accepted: July 19, 2012
Published: July 24, 2012

Adam M. Zysk, Alfred B. Garson, Qiaofeng Xu, Eric M. Brey, Wei Zhou, Jovan G. Brankov, Miles N. Wernick, Jerome R. Kuszak, and Mark A. Anastasio, "Nondestructive volumetric imaging of tissue microstructure with benchtop x-ray phase-contrast tomography and critical point drying," Biomed. Opt. Express 3, 1924-1932 (2012)

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