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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2004–2011

Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope

David G. Winters, David R. Smith, Philip Schlup, and Randy A. Bartels  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 9, pp. 2004-2011 (2012)
http://dx.doi.org/10.1364/BOE.3.002004


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Abstract

Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy. The three-dimensional (3D) polarization is gathered by measurement of a series of holograms for which excitation and analyzer polarizations are systematically varied, and the 3D SHG field is recovered through numerical back propagation. Harmonophore orientation is resolved in 3D from a sub-set of polarization-resolved SHG holograms. We further expand on previous approaches for the determination of susceptibility ratios, adding the calculation of multiple ratio values to allow intrinsic verification.

© 2012 OSA

ToC Category:
Microscopy

History
Original Manuscript: June 13, 2012
Revised Manuscript: July 30, 2012
Manuscript Accepted: July 31, 2012
Published: August 3, 2012

Citation
David G. Winters, David R. Smith, Philip Schlup, and Randy A. Bartels, "Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope," Biomed. Opt. Express 3, 2004-2011 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-9-2004


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