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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2111–2120

Cell death detection by quantitative three-dimensional single-cell tomography

Nai-Chia Cheng, Tsung-Hsun Hsieh, Yu-Ta Wang, Chien-Chih Lai, Chia-Kai Chang, Ming-Yi Lin, Ding-Wei Huang, Jeng-Wei Tjiu, and Sheng-Lung Huang  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 9, pp. 2111-2120 (2012)

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Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level.

© 2012 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1870) Medical optics and biotechnology : Dermatology
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Cell Studies

Original Manuscript: June 13, 2012
Revised Manuscript: July 24, 2012
Manuscript Accepted: August 2, 2012
Published: August 13, 2012

Nai-Chia Cheng, Tsung-Hsun Hsieh, Yu-Ta Wang, Chien-Chih Lai, Chia-Kai Chang, Ming-Yi Lin, Ding-Wei Huang, Jeng-Wei Tjiu, and Sheng-Lung Huang, "Cell death detection by quantitative three-dimensional single-cell tomography," Biomed. Opt. Express 3, 2111-2120 (2012)

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