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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2184–2189

Short-coherence off-axis holographic phase microscopy of live cell dynamics

Stefan Witte, Andrius Plauşka, Margreet C. Ridder, Laura van Berge, Huibert D. Mansvelder, and Marie Louise Groot  »View Author Affiliations


Biomedical Optics Express, Vol. 3, Issue 9, pp. 2184-2189 (2012)
http://dx.doi.org/10.1364/BOE.3.002184


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Abstract

We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.

© 2012 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1650) Medical optics and biotechnology : Coherence imaging
(320.7160) Ultrafast optics : Ultrafast technology
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: June 14, 2012
Revised Manuscript: August 22, 2012
Manuscript Accepted: August 22, 2012
Published: August 22, 2012

Virtual Issues
BIOMED 2012 (2012) Biomedical Optics Express

Citation
Stefan Witte, Andrius Plauşka, Margreet C. Ridder, Laura van Berge, Huibert D. Mansvelder, and Marie Louise Groot, "Short-coherence off-axis holographic phase microscopy of live cell dynamics," Biomed. Opt. Express 3, 2184-2189 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-9-2184


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