OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2184–2189

Short-coherence off-axis holographic phase microscopy of live cell dynamics

Stefan Witte, Andrius Plauşka, Margreet C. Ridder, Laura van Berge, Huibert D. Mansvelder, and Marie Louise Groot  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 9, pp. 2184-2189 (2012)

View Full Text Article

Enhanced HTML    Acrobat PDF (1441 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.

© 2012 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1650) Medical optics and biotechnology : Coherence imaging
(320.7160) Ultrafast optics : Ultrafast technology
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: June 14, 2012
Revised Manuscript: August 22, 2012
Manuscript Accepted: August 22, 2012
Published: August 22, 2012

Virtual Issues
BIOMED 2012 (2012) Biomedical Optics Express

Stefan Witte, Andrius Plauşka, Margreet C. Ridder, Laura van Berge, Huibert D. Mansvelder, and Marie Louise Groot, "Short-coherence off-axis holographic phase microscopy of live cell dynamics," Biomed. Opt. Express 3, 2184-2189 (2012)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science248(4951), 73–76 (1990). [CrossRef] [PubMed]
  2. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science254(5035), 1178–1181 (1991). [CrossRef] [PubMed]
  3. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett.24(5), 291–293 (1999). [CrossRef] [PubMed]
  4. U. Schnars and W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol.13(9), R85–R101 (2002). [CrossRef]
  5. W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, “Digital in-line holography for biological applications,” Proc. Natl. Acad. Sci. U.S.A.98(20), 11301–11305 (2001). [CrossRef] [PubMed]
  6. P. Massatsch, F. Charrière, E. Cuche, P. Marquet, and C. D. Depeursinge, “Time-domain optical coherence tomography with digital holographic microscopy,” Appl. Opt.44(10), 1806–1812 (2005). [CrossRef] [PubMed]
  7. G. Pedrini and H. J. Tiziani, “Short-coherence digital microscopy by use of a lensless holographic imaging system,” Appl. Opt.41(22), 4489–4496 (2002). [CrossRef] [PubMed]
  8. Z. Wang, I. S. Chun, X. Li, Z.-Y. Ong, E. Pop, L. Millet, M. Gillette, and G. Popescu, “Topography and refractometry of nanostructures using spatial light interference microscopy,” Opt. Lett.35(2), 208–210 (2010). [CrossRef] [PubMed]
  9. B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett.37(6), 1094–1096 (2012). [CrossRef] [PubMed]
  10. E. N. Leith and J. Upatnieks, “Reconstructed wavefronts and communication theory,” J. Opt. Soc. Am.52(10), 1123–1128 (1962). [CrossRef]
  11. M. Tziraki, R. Jones, P. M. W. French, M. R. Melloch, and D. D. Nolte, “Photorefractive holography for imaging through turbid media using low coherence light,” Appl. Phys. B70(1), 151–154 (2000). [CrossRef]
  12. E. Cuche, P. Marquet, and C. Depeursinge, “Spatial filtering for zero-order and twin-image elimination in digital off-axis holography,” Appl. Opt.39(23), 4070–4075 (2000). [CrossRef] [PubMed]
  13. D. Gabor, “A new microscopic principle,” Nature161(4098), 777–778 (1948). [CrossRef] [PubMed]
  14. G. Liu and P. D. Scott, “Phase retrieval and twin-image elimination for in-line Fresnel holograms,” J. Opt. Soc. Am. A4(1), 159–165 (1987). [CrossRef]
  15. S. Lai, B. King, and M. A. Neifeld, “Wave front reconstruction by means of phase-shifting digital in-line holography,” Opt. Commun.173(1-6), 155–160 (2000). [CrossRef]
  16. A. A. Maznev, T. F. Crimmins, and K. A. Nelson, “How to make femtosecond pulses overlap,” Opt. Lett.23(17), 1378–1380 (1998). [CrossRef] [PubMed]
  17. Z. Ansari, Y. Gu, M. Tziraki, R. Jones, P. M. W. French, D. D. Nolte, and M. R. Melloch, “Elimination of beam walk-off in low-coherence off-axis photorefractive holography,” Opt. Lett.26(6), 334–336 (2001). [CrossRef] [PubMed]
  18. M. C. Ridder, I. Boor, J. C. Lodder, N. L. Postma, X. Capdevila-Nortes, A. Duarri, A. B. Brussaard, R. Estévez, G. C. Scheper, H. D. Mansvelder, and M. S. van der Knaap, “Megalencephalic leucoencephalopathy with cysts: defect in chloride currents and cell volume regulation,” Brain134(11), 3342–3354 (2011). [CrossRef] [PubMed]
  19. H. Inoue, S.-I. Mori, S. Morishima, and Y. Okada, “Volume-sensitive chloride channels in mouse cortical neurons: characterization and role in volume regulation,” Eur. J. Neurosci.21(6), 1648–1658 (2005). [CrossRef] [PubMed]
  20. Y. Okada, T. Shimizu, E. Maeno, S. Tanabe, X. Wang, and N. Takahashi, “Volume-sensitive chloride channels involved in apoptotic volume decrease and cell death,” J. Membr. Biol.209(1), 21–29 (2006). [CrossRef] [PubMed]
  21. W. E. Crowe, J. Altamirano, L. Huerto, and F. J. Alvarez-Leefmans, “Volume changes in single N1E-115 neuroblastoma cells measured with a fluorescent probe,” Neuroscience69(1), 283–296 (1995). [CrossRef] [PubMed]
  22. K. Strange, “Cellular volume homeostasis,” Adv. Physiol. Educ.28(4), 155–159 (2004). [CrossRef] [PubMed]
  23. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express13(23), 9361–9373 (2005). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited