Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

doi:10.1364/BOE.3.002190

Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

Aline Pellizzaro, Gabriel Welker, David Scott, Rance Solomon, James Cooper, Anthony Farone, Mary Farone, Robert S. Mushi, Maria del Pilar Aguinaga, and Daniel Erenso »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 9, pp. 2190-2199 (2012)
http://dx.doi.org/10.1364/BOE.3.002190

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Abstract

Using a laser trap, we have studied the properties of erythrocytes from a sickle cell anemia patient (SCA) after receiving an intravenous blood transfusion, and a normal adult individual carrying normal adult hemoglobin. The hemoglobin type and quantitation assessment was carried out by high performance liquid chromatography (HPLC). We conducted an analysis of the size distributions of the cells. By targeting those erythrocytes in the overlapping regions of size distributions, we have investigated their properties when the cells are trapped and released. The efficacy of the transfusion treatment is also studied by comparing the relative changes in deformation and the relaxation-time of the cells in the two samples.

OCIS Codes
(000.1430) General : Biology and medicine
(000.2190) General : Experimental physics
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Traps, Manipulation, and Tracking

History
Original Manuscript: June 18, 2012
Revised Manuscript: August 15, 2012
Manuscript Accepted: August 16, 2012
Published: August 23, 2012

Citation
Aline Pellizzaro, Gabriel Welker, David Scott, Rance Solomon, James Cooper, Anthony Farone, Mary Farone, Robert S. Mushi, Maria del Pilar Aguinaga, and Daniel Erenso, "Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient," Biomed. Opt. Express 3, 2190-2199 (2012)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-3-9-2190

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References

J. B. Herrick, “Peculiar elongated and sickle-shaped red blood corpuscles in a case of severe anemia,” Arch. Intern. Med.VI(5), 517–521 (1910). [CrossRef]
L. H. Pauling, H. A. Itano, S. J. Singer, and I. C. Wells, “Sickle cell anemia a molecular disease,” Science110(2865), 543–548 (1949). [CrossRef] [PubMed]
V. M. Ingram, “Gene mutations in human haemoglobin: the chemical difference between normal and sickle cell haemoglobin,” Nature180(4581), 326–328 (1957). [CrossRef] [PubMed]
C. Madigan and P. Malik, “Pathophysiology and therapy for haemoglobinopathies. Part I: sickle cell disease,” Expert Rev. Mol. Med.8(09), 1–23 (2006). [CrossRef] [PubMed]
M. P. Aguinaga, “Newborn screening and clinical management of children with sickle cell disease,” in J. Navarro, ed, Frontiers of Science in the 21st Century (Cordillera, Lima, 2007), pp. 137–147.
S. Lanzkron, J. J. Strouse, R. Wilson, M. C. Beach, C. Haywood, H. Park, C. Witkop, E. B. Bass, and J. B. Segal, “Systematic review: Hydroxyurea for the treatment of adults with sickle cell disease,” Ann. Intern. Med.148(12), 939–955 (2008). [PubMed]
E. Beutler, M. A. Lichtma, and B. S. Coller, “The sickle cell diseases and related disorders,” in Williams Hematology, 6th ed. (McGraw-Hill, New York, 2001). pp. 581–606.
A. Kutlar, F. Kutlar, J. B. Wilson, M. G. Headlee, and T. H. J. Huisman, “Quantitation of hemoglobin components by high-performance cation-exchange liquid chromatography: its use in diagnosis and in the assessment of cellular distribution of hemoglobin variants,” Am. J. Hematol.17(1), 39–53 (1984). [CrossRef] [PubMed]
A. Ashkin, “Applications of laser radiation pressure,” Science210(4474), 1081–1088 (1980). [CrossRef] [PubMed]
A. Ashkin, J. M. Dziedzic, and T. Yamane, “Optical trapping and manipulation of single cells using infrared laser beams,” Nature330(6150), 769–771 (1987). [CrossRef] [PubMed]
F. Bordeleau, J. Bessard, N. Marceau, and Y. Sheng, “Measuring integrated cellular mechanical stress response at focal adhesions by optical tweezers,” J. Biomed. Opt.16(9), 095005 (2011). [CrossRef] [PubMed]
S. Rancourt-Grenier, M. T. Wei, J. J. Bai, A. Chiou, P. P. Bareil, P. L. Duval, and Y. Sheng, “Dynamic deformation of red blood cell in dual-trap optical tweezers,” Opt. Express18(10), 10462–10472 (2010). [CrossRef] [PubMed]
D. Erenso, A. Shulman, J. Curtis, and S. Elrod, “Formation of synthetic structures with micron size silica beads using optical tweezer,” J. Mod. Opt.54(10), 1529–1536 (2007). [CrossRef]
M. M. Brandão, A. Fontes, M. L. Barjas-Castro, L. C. Barbosa, F. F. Costa, C. L. Cesar, and S. T. Saad, “Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease,” Eur. J. Haematol.70(4), 207–211 (2003). [CrossRef] [PubMed]
M. M. Brandão, S. T. O. Saad, C. L. Cezar, A. Fontes, F. F. Costa, and M. L. Barjas-Castro, “Elastic properties of stored red blood cells from sickle trait donor units,” Vox Sang.85(3), 213–215 (2003). [CrossRef] [PubMed]
G. B. Liao, P. B. Bareil, Y. Sheng, and A. Chiou, “One-dimensional jumping optical tweezers for optical stretching of bi-concave human red blood cells,” Opt. Express16(3), 1996–2004 (2008). [CrossRef] [PubMed]
G. Athanassiou, A. Moutzouri, A. Kourakli, and N. Zoumbos, “Effect of hydroxyurea on the deformability of the red blood cell membrane in patients with sickle cell anemia,” Clin. Hemorheol. Microcirc.35(1-2), 291–295 (2006). [PubMed]
I. Dulińska, M. Targosz, W. Strojny, M. Lekka, P. Czuba, W. Balwierz, and M. Szymoński, “Stiffness of normal and pathological erythrocytes studied by means of atomic force microscopy,” J. Biochem. Biophys. Methods66(1-3), 1–11 (2006). [CrossRef] [PubMed]
J. L. Maciaszek, B. Andemariam, and G. Lykotrafitis, “Microelasticity of red blood cells in sickle cell disease,” J. Strain Analysis Eng. Design46(5), 368–379 (2011). [CrossRef]

Andemariam, B.
Ashkin, A.
Athanassiou, G.
Bai, J. J.
Balwierz, W.
Barbosa, L. C.
Bareil, P. B.
Bareil, P. P.
Barjas-Castro, M. L.
Bass, E. B.
Beach, M. C.
Bessard, J.
Bordeleau, F.
Brandão, M. M.
Cesar, C. L.
Cezar, C. L.
Chiou, A.
Costa, F. F.
Curtis, J.
Czuba, P.
Dulinska, I.
Duval, P. L.
Dziedzic, J. M.
Elrod, S.
Erenso, D.
Fontes, A.
Haywood, C.
Headlee, M. G.
Herrick, J. B.
Huisman, T. H. J.
Ingram, V. M.
Itano, H. A.
Kourakli, A.
Kutlar, A.
Kutlar, F.
Lanzkron, S.
Lekka, M.
Liao, G. B.
Lykotrafitis, G.
Maciaszek, J. L.
Madigan, C.
Malik, P.
Marceau, N.
Moutzouri, A.
Park, H.
Pauling, L. H.
Rancourt-Grenier, S.
Saad, S. T.
Saad, S. T. O.
Segal, J. B.
Sheng, Y.
Shulman, A.
Singer, S. J.
Strojny, W.
Strouse, J. J.
Szymonski, M.
Targosz, M.
Wei, M. T.
Wells, I. C.
Wilson, J. B.
Wilson, R.
Witkop, C.
Yamane, T.
Zoumbos, N.

Am. J. Hematol.

A. Kutlar, F. Kutlar, J. B. Wilson, M. G. Headlee, and T. H. J. Huisman, “Quantitation of hemoglobin components by high-performance cation-exchange liquid chromatography: its use in diagnosis and in the assessment of cellular distribution of hemoglobin variants,” Am. J. Hematol.17(1), 39–53 (1984). [CrossRef] [PubMed]

Ann. Intern. Med.

S. Lanzkron, J. J. Strouse, R. Wilson, M. C. Beach, C. Haywood, H. Park, C. Witkop, E. B. Bass, and J. B. Segal, “Systematic review: Hydroxyurea for the treatment of adults with sickle cell disease,” Ann. Intern. Med.148(12), 939–955 (2008). [PubMed]

Arch. Intern. Med.

J. B. Herrick, “Peculiar elongated and sickle-shaped red blood corpuscles in a case of severe anemia,” Arch. Intern. Med.VI(5), 517–521 (1910). [CrossRef]

Clin. Hemorheol. Microcirc.

G. Athanassiou, A. Moutzouri, A. Kourakli, and N. Zoumbos, “Effect of hydroxyurea on the deformability of the red blood cell membrane in patients with sickle cell anemia,” Clin. Hemorheol. Microcirc.35(1-2), 291–295 (2006). [PubMed]

Eur. J. Haematol.

M. M. Brandão, A. Fontes, M. L. Barjas-Castro, L. C. Barbosa, F. F. Costa, C. L. Cesar, and S. T. Saad, “Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease,” Eur. J. Haematol.70(4), 207–211 (2003). [CrossRef] [PubMed]

Expert Rev. Mol. Med.

C. Madigan and P. Malik, “Pathophysiology and therapy for haemoglobinopathies. Part I: sickle cell disease,” Expert Rev. Mol. Med.8(09), 1–23 (2006). [CrossRef] [PubMed]

J. Biochem. Biophys. Methods

I. Dulińska, M. Targosz, W. Strojny, M. Lekka, P. Czuba, W. Balwierz, and M. Szymoński, “Stiffness of normal and pathological erythrocytes studied by means of atomic force microscopy,” J. Biochem. Biophys. Methods66(1-3), 1–11 (2006). [CrossRef] [PubMed]

J. Biomed. Opt.

F. Bordeleau, J. Bessard, N. Marceau, and Y. Sheng, “Measuring integrated cellular mechanical stress response at focal adhesions by optical tweezers,” J. Biomed. Opt.16(9), 095005 (2011). [CrossRef] [PubMed]

J. Mod. Opt.

D. Erenso, A. Shulman, J. Curtis, and S. Elrod, “Formation of synthetic structures with micron size silica beads using optical tweezer,” J. Mod. Opt.54(10), 1529–1536 (2007). [CrossRef]

J. Strain Analysis Eng. Design

J. L. Maciaszek, B. Andemariam, and G. Lykotrafitis, “Microelasticity of red blood cells in sickle cell disease,” J. Strain Analysis Eng. Design46(5), 368–379 (2011). [CrossRef]

Nature

V. M. Ingram, “Gene mutations in human haemoglobin: the chemical difference between normal and sickle cell haemoglobin,” Nature180(4581), 326–328 (1957). [CrossRef] [PubMed]
A. Ashkin, J. M. Dziedzic, and T. Yamane, “Optical trapping and manipulation of single cells using infrared laser beams,” Nature330(6150), 769–771 (1987). [CrossRef] [PubMed]

Opt. Express

Science

L. H. Pauling, H. A. Itano, S. J. Singer, and I. C. Wells, “Sickle cell anemia a molecular disease,” Science110(2865), 543–548 (1949). [CrossRef] [PubMed]
A. Ashkin, “Applications of laser radiation pressure,” Science210(4474), 1081–1088 (1980). [CrossRef] [PubMed]

Vox Sang.

M. M. Brandão, S. T. O. Saad, C. L. Cezar, A. Fontes, F. F. Costa, and M. L. Barjas-Castro, “Elastic properties of stored red blood cells from sickle trait donor units,” Vox Sang.85(3), 213–215 (2003). [CrossRef] [PubMed]

Other

E. Beutler, M. A. Lichtma, and B. S. Coller, “The sickle cell diseases and related disorders,” in Williams Hematology, 6th ed. (McGraw-Hill, New York, 2001). pp. 581–606.
M. P. Aguinaga, “Newborn screening and clinical management of children with sickle cell disease,” in J. Navarro, ed, Frontiers of Science in the 21st Century (Cordillera, Lima, 2007), pp. 137–147.

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