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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 3, Iss. 9 — Sep. 1, 2012
  • pp: 2306–2316

Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin

Franklin Tellier, Jérôme Steibel, Renée Chabrier, François Xavier Blé, Hervé Tubaldo, Ravelo Rasata, Jacques Chambron, Guy Duportail, Hervé Simon, Jean-François Rodier, and Patrick Poulet  »View Author Affiliations

Biomedical Optics Express, Vol. 3, Issue 9, pp. 2306-2316 (2012)

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Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10−4 to 1.7 × 10−2, which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L−1 was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L−1 range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals.

© 2012 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Optics in Cancer Research

Original Manuscript: May 31, 2012
Revised Manuscript: July 20, 2012
Manuscript Accepted: July 27, 2012
Published: August 30, 2012

Franklin Tellier, Jérôme Steibel, Renée Chabrier, François Xavier Blé, Hervé Tubaldo, Ravelo Rasata, Jacques Chambron, Guy Duportail, Hervé Simon, Jean-François Rodier, and Patrick Poulet, "Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin," Biomed. Opt. Express 3, 2306-2316 (2012)

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  1. D. N. Krag, D. L. Weaver, J. C. Alex, and J. T. Fairbank, “Surgical resection and radiolocalization of the sentinel lymph node in breast cancer using a gamma probe,” Surg. Oncol.2(6), 335–340 (1993). [CrossRef] [PubMed]
  2. A. E. Giuliano, D. M. Kirgan, J. M. Guenther, and D. L. Morton, “Lymphatic mapping and sentinel lymphadenectomy for breast cancer,” Ann. Surg.220(3), 391–401 (1994). [CrossRef] [PubMed]
  3. G. Mariani, L. Moresco, G. Viale, G. Villa, M. Bagnasco, G. Canavese, J. Buscombe, H. W. Strauss, and G. Paganelli, “Radioguided sentinel lymph node biopsy in breast cancer surgery,” J. Nucl. Med.42(8), 1198–1215 (2001). [PubMed]
  4. J. F. Rodier, M. Velten, M. Wilt, P. Martel, G. Ferron, V. Vaini-Elies, H. Mignotte, A. Brémond, J. M. Classe, F. Dravet, T. Routiot, C. T. de Lara, A. Avril, G. Lorimier, E. Fondrinier, G. Houvenaeghel, and S. Avigdor, “Prospective multicentric randomized study comparing periareolar and peritumoral injection of radiotracer and blue dye for the detection of sentinel lymph node in breast sparing procedures: FRANSENODE trial,” J. Clin. Oncol.25(24), 3664–3669 (2007). [CrossRef] [PubMed]
  5. B. E. Schaafsma, J. S. Mieog, M. Hutteman, J. R. van der Vorst, P. J. Kuppen, C. W. Löwik, J. V. Frangioni, C. J. van de Velde, and A. L. Vahrmeijer, “The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery,” J. Surg. Oncol.104(3), 323–332 (2011). [CrossRef] [PubMed]
  6. T. Sugie, K. A. Kassim, M. Takeuchi, T. Hashimoto, K. Yamagami, Y. Masai, and M. Toi, “A novel method for sentinel lymph node biopsy by indocyanine green fluorescence technique in breast cancer,” Cancers2(2), 713–720 (2010). [CrossRef]
  7. M. Noguchi, M. Noguchi, Y. Nakano, Y. Ohno, and T. Kosaka, “Axillary reverse mapping using a fluorescence imaging system in breast cancer,” J. Surg. Oncol.105(3), 229–234 (2012). [CrossRef] [PubMed]
  8. C. Hirche, Z. Mohr, S. Kneif, D. Murawa, and M. Hünerbein, “High rate of solitary sentinel node metastases identification by fluorescence-guided lymphatic imaging in breast cancer,” J. Surg. Oncol.105(2), 162–166 (2012). [CrossRef] [PubMed]
  9. T. Hojo, T. Nagao, M. Kikuyama, S. Akashi, and T. Kinoshita, “Evaluation of sentinel node biopsy by combined fluorescent and dye method and lymph flow for breast cancer,” Breast19(3), 210–213 (2010). [CrossRef] [PubMed]
  10. M. Hutteman, J. S. D. Mieog, J. R. van der Vorst, G. J. Liefers, H. Putter, C. W. G. M. Löwik, J. V. Frangioni, C. J. H. van de Velde, and A. L. Vahrmeijer, “Randomized, double-blind comparison of indocyanine green with or without albumin premixing for near-infrared fluorescence imaging of sentinel lymph nodes in breast cancer patients,” Breast Cancer Res. Treat.127(1), 163–170 (2011). [CrossRef] [PubMed]
  11. A. C. van Leeuwen, T. Buckle, G. Bendle, L. Vermeeren, R. Valdés Olmos, H. G. van de Poel, and F. W. van Leeuwen, “Tracer-cocktail injections for combined pre- and intraoperative multimodal imaging of lymph nodes in a spontaneous mouse prostate tumor model,” J. Biomed. Opt.16(1), 016004 (2011). [CrossRef] [PubMed]
  12. C. Kim, T. N. Erpelding, L. Jankovic, and L. V. Wang, “Performance benchmarks of an array-based hand-help photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging,” Philos. Trans. R. Soc. A Math. Phys. Eng. Sci.369(1955), 4644–4650 (2011). [CrossRef]
  13. T. N. Erpelding, C. Kim, M. Pramanik, L. Jankovic, K. Maslov, Z. Guo, J. A. Margenthaler, M. D. Pashley, and L. V. Wang, “Sentinel lymph nodes in the rat: noninvasive photoacoustic and US imaging with a clinical US system,” Radiology256(1), 102–110 (2010). [CrossRef] [PubMed]
  14. C. Kim, K. H. Song, F. Gao, and L. V. Wang, “Sentinel lymph nodes and lymphatic vessels: noninvasive dual-modality in vivo mapping by using indocyanine green in rats—volumetric spectroscopic photoacoustic imaging and planar fluorescence imaging,” Radiology255(2), 442–450 (2010). [CrossRef] [PubMed]
  15. J. V. Frangioni, S. W. Kim, S. Ohnishi, S. Kim, and M. G. Bawendi, “Sentinel lymph node mapping with type-II quantum dots,” Methods Mol. Biol.374, 147–159 (2007). [PubMed]
  16. E. Pic, T. Pons, L. Bezdetnaya, A. Leroux, F. Guillemin, B. Dubertret, and F. Marchal, “Fluorescence imaging and whole-body biodistribution of near-infrared-emitting quantum dots after subcutaneous injection for regional lymph node mapping in mice,” Mol. Imaging Biol.12(4), 394–405 (2010). [CrossRef] [PubMed]
  17. T. Buckle, A. C. van Leeuwen, P. T. Chin, H. Janssen, S. H. Muller, J. Jonkers, and F. W. van Leeuwen, “A self-assembled multimodal complex for combined pre- and intraoperative imaging of the sentinel lymph node,” Nanotechnology21(35), 355101 (2010). [CrossRef] [PubMed]
  18. Y. H. Jeon, Y. H. Kim, K. Choi, J. Y. Piao, B. Quan, Y. S. Lee, J. M. Jeong, J. K. Chung, D. S. Lee, M. C. Lee, J. Lee, D. S. Chung, and K. W. Kang, “In vivo imaging of sentinel nodes using fluorescent silica nanoparticles in living mice,” Mol. Imaging Biol.12(2), 155–162 (2010). [CrossRef] [PubMed]
  19. W. J. Akers, C. Kim, M. Berezin, K. Guo, R. Fuhrhop, G. M. Lanza, G. M. Fischer, E. Daltrozzo, A. Zumbusch, X. Cai, L. V. Wang, and S. Achilefu, “Noninvasive photoacoustic and fluorescence sentinel lymph node identification using dye-loaded perfluorocarbon nanoparticles,” ACS Nano5(1), 173–182 (2011). [CrossRef] [PubMed]
  20. F. Tellier, R. Ravelo, H. Simon, R. Chabrier, J. Steibel, and P. Poulet, “Sentinel lymph node detection by an optical method using scattered photons,” Biomed. Opt. Express1(3), 902–910 (2010). [CrossRef] [PubMed]
  21. F. Tellier, H. Simon, F. X. Ble, R. Ravelo, R. Chabrier, J. Steibel, J. F. Rodier, and P. Poulet, “Comparison of 2- and 4-wavelength methods for the optical detection of sentinel lymph node,” Proc. SPIE8092, 80920L, 80920L-7 (2011). [CrossRef]
  22. C. Tsopelas and R. Sutton, “Why certain dyes are useful for localizing the sentinel lymph node,” J. Nucl. Med.43(10), 1377–1382 (2002). [PubMed]
  23. D. W. Newton, P. J. Breen, D. E. Brown, J. F. Mackie, and R. B. Kluza, “Physicochemical characteristics of patent blue violet dye,” J. Pharm. Sci.70(2), 122–127 (1981). [CrossRef] [PubMed]
  24. M. S. Baptista and G. L. Indig, “Effect of BSA binding on photophysical and photochemical properties of triarylmethane dyes,” J. Phys. Chem. B102(23), 4678–4688 (1998). [CrossRef]
  25. C. H. Sloop, L. Dory, and P. S. Roheim, “Interstitial fluid lipoproteins,” J. Lipid Res.28(3), 225–237 (1987). [PubMed]
  26. S. T. Flock, S. L. Jacques, B. C. Wilson, W. M. Star, and M. J. C. van Gemert, “Optical properties of Intralipid: a phantom medium for light propagation studies,” Lasers Surg. Med.12(5), 510–519 (1992). [CrossRef] [PubMed]
  27. T. Svensson, J. Swartling, P. Taroni, A. Torricelli, P. Lindblom, C. Ingvar, and S. Andersson-Engels, “Characterization of normal breast tissue heterogeneity using time-resolved near-infrared spectroscopy,” Phys. Med. Biol.50(11), 2559–2571 (2005). [CrossRef] [PubMed]
  28. H. W. Melhuish and W. S. Metcalf, “The quenching of the fluorescence of anthracene; the transition from strong to weak quenching,” J. Chem. Soc.976–979 (1954). [CrossRef]
  29. H. Du, R. A. Fuh, J. Li, L. A. Corkan, and J. S. Lindsey, “PhotochemCAD++: a computer-aided design and research tool in photochemistry,” Photochem. Photobiol.68(2), 141–142 (1998).
  30. I. J. Namaer, J. Steibel, P. Poulet, J. P. Armspach, Y. Mauss, and J. Chambron, “In vivo dynamic MR imaging of MBP-induced acute experimental allergic encephalomyelitis in Lewis rat,” Magn. Reson. Med.24(2), 325–334 (1992). [CrossRef] [PubMed]
  31. N. L. Tilney, “Patterns of lymphatic drainage in the adult laboratory rat,” J. Anat.109(Pt 3), 369–383 (1971). [PubMed]
  32. J. R. Babendure, S. R. Adams, and R. Y. Tsien, “Aptamers switch on fluorescence of triphenylmethane dyes,” J. Am. Chem. Soc.125(48), 14716–14717 (2003). [CrossRef] [PubMed]
  33. A. Werner, P. V. Konarev, D. I. Svergun, and U. Hahn, “Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering,” Anal. Biochem.389(1), 52–62 (2009). [CrossRef] [PubMed]
  34. J. A. Bartlett and G. L. Indig, “Effect of self-association and protein binding on the photochemical reactivity of triarylmethanes. Implications of noncovalent interactions on the competition between photosensitization mechanisms type I and type II,” Photochem. Photobiol.70(4), 490–498 (1999). [CrossRef] [PubMed]
  35. V. J. Pansare, S. Hejazi, W. J. Faenza, and R. K. Prud’homme, “Review of long-wavelength optical and NIR imaging materials contrast agents, fluorophores, and multifunctional nano carriers,” Chem. Mater.24(5), 812–827 (2012). [CrossRef]
  36. T. J. Russin, E. I. Altınoğlu, J. H. Adair, and P. C. Eklund, “Measuring the fluorescent quantum efficiency of indocyanine green encapsulated in nanocomposite particulates,” J. Phys. Condens. Matter22(33), 334217 (2010). [CrossRef] [PubMed]
  37. C. E. Metz, “Basic principles of ROC analysis,” Semin. Nucl. Med.8(4), 283–298 (1978). [CrossRef] [PubMed]
  38. F. Tellier, P. Poulet, J. P. Ghnassia, M. Wilt, D. Weitbruch, and J. F. Rodier, “A new optical probe for the detection of the sentinel lymph node using Patent Blue V dye in breast cancer: a preliminary study,” Mol. Clin. Oncol. (to be published).
  39. L. Barthelmes, A. Goyal, R. G. Newcombe, F. McNeill, R. E. Mansel, and NEW START and ALMANAC study groups, “Adverse reactions to patent blue V dye—The NEW START and ALMANAC experience,” Eur. J. Surg. Oncol.36(4), 399–403 (2010). [CrossRef] [PubMed]

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