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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 1 — Jan. 1, 2013
  • pp: 89–104

Effect of signal intensity and camera quantization on laser speckle contrast analysis

Lipei Song and Daniel S. Elson  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 1, pp. 89-104 (2013)
http://dx.doi.org/10.1364/BOE.4.000089


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Abstract

Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of the signals by the camera and analog-to-digital converter (ADC). In this paper we deduce the theoretical relationship between signal intensity and contrast values based on the probability density function (PDF) of the speckle pattern and simplify it to a rational function. A simple method to correct this contrast error is suggested. The experimental results demonstrate that this relationship can effectively compensate the bias in contrast values induced by the quantized signal intensity and correct for bias induced by signal intensity variations across the field of view.

© 2012 OSA

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Speckle Imaging and Diagnostics

History
Original Manuscript: September 17, 2012
Revised Manuscript: November 5, 2012
Manuscript Accepted: November 28, 2012
Published: December 13, 2012

Citation
Lipei Song and Daniel S. Elson, "Effect of signal intensity and camera quantization on laser speckle contrast analysis," Biomed. Opt. Express 4, 89-104 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-1-89


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