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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1846–1855

In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Mengyang Liu, Nicole Schmitner, Michelle G. Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, and Wolfgang Drexler  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 10, pp. 1846-1855 (2013)
http://dx.doi.org/10.1364/BOE.4.001846


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Abstract

For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Photoacoustic Imaging and Spectroscopy

History
Original Manuscript: July 22, 2013
Revised Manuscript: August 22, 2013
Manuscript Accepted: August 24, 2013
Published: August 29, 2013

Citation
Mengyang Liu, Nicole Schmitner, Michelle G. Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, and Wolfgang Drexler, "In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish," Biomed. Opt. Express 4, 1846-1855 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-10-1846


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