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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1846–1855

In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Mengyang Liu, Nicole Schmitner, Michelle G. Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, and Wolfgang Drexler  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 10, pp. 1846-1855 (2013)

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For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.

© 2013 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Photoacoustic Imaging and Spectroscopy

Original Manuscript: July 22, 2013
Revised Manuscript: August 22, 2013
Manuscript Accepted: August 24, 2013
Published: August 29, 2013

Mengyang Liu, Nicole Schmitner, Michelle G. Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, and Wolfgang Drexler, "In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish," Biomed. Opt. Express 4, 1846-1855 (2013)

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  1. B. W. Rice and C. H. Contag, “The importance of being red,” Nat. Biotechnol.27(7), 624–625 (2009). [CrossRef] [PubMed]
  2. J. W. Lichtman and J.-A. Conchello, “Fluorescence microscopy,” Nat. Methods2(12), 910–919 (2005). [CrossRef] [PubMed]
  3. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy,” Science305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
  4. H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods4(4), 331–336 (2007). [CrossRef] [PubMed]
  5. J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science296(5567), 541–545 (2002). [CrossRef] [PubMed]
  6. J. McGinty, H. B. Taylor, L. Chen, L. Bugeon, J. R. Lamb, M. J. Dallman, and P. M. French, “In vivo fluorescence lifetime optical projection tomography,” Biomed. Opt. Express2(5), 1340–1350 (2011). [CrossRef] [PubMed]
  7. P. Beard, “Biomedical photoacoustic imaging,” Interface Focus1(4), 602–631 (2011). [CrossRef] [PubMed]
  8. L. V. Wang and H.-i. Wu, Biomedical Optics: Principles and Imaging (Wiley-Interscience, Hoboken, N.J., 2007).
  9. E. Zhang and P. Beard, “Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control53(7), 1330–1338 (2006). [CrossRef] [PubMed]
  10. E. Zhang, J. Laufer, and P. Beard, “Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues,” Appl. Opt.47(4), 561–577 (2008). [CrossRef] [PubMed]
  11. J. Laufer, P. Johnson, E. Zhang, B. Treeby, B. Cox, B. Pedley, and P. Beard, “In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy,” J. Biomed. Opt.17(5), 056016 (2012). [CrossRef] [PubMed]
  12. J. Laufer, F. Norris, J. Cleary, E. Zhang, B. Treeby, B. Cox, P. Johnson, P. Scambler, M. Lythgoe, and P. Beard, “In vivo photoacoustic imaging of mouse embryos,” J. Biomed. Opt.17(6), 061220 (2012). [CrossRef] [PubMed]
  13. B. Cox, J. G. Laufer, S. R. Arridge, and P. C. Beard, “Quantitative spectroscopic photoacoustic imaging: a review,” J. Biomed. Opt.17(6), 061202 (2012). [CrossRef] [PubMed]
  14. M. R. Fein and M. Egeblad, “Caught in the act: revealing the metastatic process by live imaging,” Dis. Model. Mech.6(3), 580–593 (2013). [CrossRef] [PubMed]
  15. S. Liu and S. D. Leach, “Screening pancreatic oncogenes in zebrafish using the Gal4/UAS system,” Methods Cell Biol.105, 367–381 (2011). [CrossRef] [PubMed]
  16. H. R. Shive, “Zebrafish Models for Human Cancer,” Vet. Pathol.50(3), 468–482 (2013). [CrossRef] [PubMed]
  17. W. Y. Choi and K. D. Poss, “Cardiac regeneration,” Curr. Top. Dev. Biol.100, 319–344 (2012). [CrossRef] [PubMed]
  18. C. Kizil, J. Kaslin, V. Kroehne, and M. Brand, “Adult neurogenesis and brain regeneration in zebrafish,” Dev. Neurobiol.72(3), 429–461 (2012). [CrossRef] [PubMed]
  19. J. B. Moss, P. Koustubhan, M. Greenman, M. J. Parsons, I. Walter, and L. G. Moss, “Regeneration of the pancreas in adult zebrafish,” Diabetes58(8), 1844–1851 (2009). [CrossRef] [PubMed]
  20. Z. Cao, L. D. Jensen, P. Rouhi, K. Hosaka, T. Länne, J. F. Steffensen, E. Wahlberg, and Y. Cao, “Hypoxia-induced retinopathy model in adult zebrafish,” Nat. Protoc.5(12), 1903–1910 (2010). [CrossRef] [PubMed]
  21. S. Ye, R. Yang, J. Xiong, K. K. Shung, Q. Zhou, C. Li, and Q. Ren, “Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy,” Biomed. Opt. Express3(2), 360–365 (2012). [CrossRef] [PubMed]
  22. R. Ma, M. Distel, X. L. Deán-Ben, V. Ntziachristos, and D. Razansky, “Non-invasive whole-body imaging of adult zebrafish with optoacoustic tomography,” Phys. Med. Biol.57(22), 7227–7237 (2012). [CrossRef] [PubMed]
  23. C. Lee, S. Han, S. Kim, M. Jeon, M. Y. Jeon, C. Kim, and J. Kim, “Combined photoacoustic and optical coherence tomography using a single near-infrared supercontinuum laser source,” Appl. Opt.52(9), 1824–1828 (2013). [CrossRef] [PubMed]
  24. X. Cai, Y. Zhang, L. Li, S. W. Choi, M. R. MacEwan, J. Yao, C. Kim, Y. Xia, and L. V. Wang, “Investigation of neovascularization in three-dimensional porous scaffolds in vivo by a combination of multiscale photoacoustic microscopy and optical coherence tomography,” Tissue Eng. Part C Methods19(3), 196–204 (2013). [CrossRef] [PubMed]
  25. L. Xi, C. Duan, H. Xie, and H. Jiang, “Miniature probe combining optical-resolution photoacoustic microscopy and optical coherence tomography for in vivo microcirculation study,” Appl. Opt.52(9), 1928–1931 (2013). [CrossRef] [PubMed]
  26. X. Zhang, H. F. Zhang, and S. Jiao, “Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source,” J. Biomed. Opt.17(3), 030502 (2012). [CrossRef] [PubMed]
  27. S. Jiao, Z. Xie, H. F. Zhang, and C. A. Puliafito, “Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography,” Opt. Lett.34(19), 2961–2963 (2009). [CrossRef] [PubMed]
  28. L. Li, K. Maslov, G. Ku, and L. V. Wang, “Three-dimensional combined photoacoustic and optical coherence microscopy for in vivo microcirculation studies,” Opt. Express17(19), 16450–16455 (2009). [CrossRef] [PubMed]
  29. E. Z. Zhang, B. Povazay, J. Laufer, A. Alex, B. Hofer, B. Pedley, C. Glittenberg, B. Treeby, B. Cox, P. Beard, and W. Drexler, “Multimodal photoacoustic and optical coherence tomography scanner using an all optical detection scheme for 3D morphological skin imaging,” Biomed. Opt. Express2(8), 2202–2215 (2011). [CrossRef] [PubMed]
  30. A. Alex, B. Považay, B. Hofer, S. Popov, C. Glittenberg, S. Binder, and W. Drexler, “Multispectral in vivo three-dimensional optical coherence tomography of human skin,” J. Biomed. Opt.15(2), 026025 (2010). [CrossRef] [PubMed]
  31. A. Alex, J. Weingast, M. Weinigel, M. Kellner-Höfer, R. Nemecek, M. Binder, H. Pehamberger, K. König, and W. Drexler, “Three-dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology,” J Biophotonics6(4), 352–362 (2013). [CrossRef] [PubMed]
  32. American National Standards Institute, “American National Standard for Safe Use of Lasers,” ANSI Z136.1 2007–1 (2007).
  33. H. Wan, S. Korzh, Z. Li, S. P. Mudumana, V. Korzh, Y.-J. Jiang, S. Lin, and Z. Gong, “Analyses of pancreas development by generation of gfp transgenic zebrafish using an exocrine pancreas-specific elastaseA gene promoter,” Exp. Cell Res.312(9), 1526–1539 (2006). [CrossRef] [PubMed]
  34. N. Furukawa, M. Saito, T. Hakoshima, and K. Kohno, “A Diphtheria Toxin Receptor Deficient in Epidermal Growth Factor-Like Biological Activity,” J. Biochem.140(6), 831–841 (2006). [CrossRef] [PubMed]
  35. M. Westerfield, The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio Rerio). 4th ed. (Eugene: University of Oregon Press, 2000).
  36. J. Laufer, A. Jathoul, M. Pule, and P. Beard, “Evaluation of genetically expressed absorbing proteins using photoacoustic spectroscopy,” Proc. SPIE8581, 85810X, 85810X-6 (2013). [CrossRef]
  37. B. E. Treeby and B. T. Cox, “k-Wave: MATLAB toolbox for the simulation and reconstruction of photoacoustic wave fields,” J. Biomed. Opt.15(2), 021314 (2010). [CrossRef] [PubMed]
  38. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics3(7), 412–417 (2009). [CrossRef]
  39. G. S. Filonov, A. Krumholz, J. Xia, J. Yao, L. V. Wang, and V. V. Verkhusha, “Deep-Tissue Photoacoustic Tomography of a Genetically Encoded Near-Infrared Fluorescent Probe,” Angew. Chem.51(6), 1448–1451 (2012). [CrossRef] [PubMed]
  40. K. H. Song and L. V. Wang, “Deep reflection-mode photoacoustic imaging of biological tissue,” J. Biomed. Opt.12(6), 060503 (2007). [CrossRef] [PubMed]
  41. C. Huang, L. Nie, R. W. Schoonover, Z. Guo, C. O. Schirra, M. A. Anastasio, and L. V. Wang, “Aberration correction for transcranial photoacoustic tomography of primates employing adjunct image data,” J. Biomed. Opt.17(6), 066016 (2012). [CrossRef] [PubMed]
  42. L. Nie, X. Cai, K. Maslov, A. Garcia-Uribe, M. A. Anastasio, and L. V. Wang, “Photoacoustic tomography through a whole adult human skull with a photon recycler,” J. Biomed. Opt.17(11), 110506 (2012). [CrossRef] [PubMed]
  43. A. Kumar, W. Drexler, and R. A. Leitgeb, “Subaperture correlation based digital adaptive optics for full field optical coherence tomography,” Opt. Express21(9), 10850–10866 (2013). [CrossRef] [PubMed]

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