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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1925–1936

Endoscopic probe optics for spectrally encoded confocal microscopy

DongKyun Kang, Robert W. Carruth, Minkyu Kim, Simon C. Schlachter, Milen Shishkov, Kevin Woods, Nima Tabatabaei, Tao Wu, and Guillermo J. Tearney  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 10, pp. 1925-1936 (2013)

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Spectrally encoded confocal microscopy (SECM) is a form of reflectance confocal microscopy that can achieve high imaging speeds using relatively simple probe optics. Previously, the feasibility of conducting large-area SECM imaging of the esophagus in bench top setups has been demonstrated. Challenges remain, however, in translating SECM into a clinically-useable device; the tissue imaging performance should be improved, and the probe size needs to be significantly reduced so that it can fit into luminal organs of interest. In this paper, we report the development of new SECM endoscopic probe optics that addresses these challenges. A custom water-immersion aspheric singlet (NA = 0.5) was developed and used as the objective lens. The water-immersion condition was used to reduce the spherical aberrations and specular reflection from the tissue surface, which enables cellular imaging of the tissue deep below the surface. A custom collimation lens and a small-size grating were used along with the custom aspheric singlet to reduce the probe size. A dual-clad fiber was used to provide both the single- and multi- mode detection modes. The SECM probe optics was made to be 5.85 mm in diameter and 30 mm in length, which is small enough for safe and comfortable endoscopic imaging of the gastrointestinal tract. The lateral resolution was 1.8 and 2.3 µm for the single- and multi- mode detection modes, respectively, and the axial resolution 11 and 17 µm. SECM images of the swine esophageal tissue demonstrated the capability of this device to enable the visualization of characteristic cellular structural features, including basal cell nuclei and papillae, down to the imaging depth of 260 µm. These results suggest that the new SECM endoscopic probe optics will be useful for imaging large areas of the esophagus at the cellular scale in vivo.

© 2013 OSA

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.4730) Medical optics and biotechnology : Optical pathology

ToC Category:
Endoscopes, Catheters and Micro-Optics

Original Manuscript: July 22, 2013
Revised Manuscript: August 28, 2013
Manuscript Accepted: August 28, 2013
Published: September 3, 2013

DongKyun Kang, Robert W. Carruth, Minkyu Kim, Simon C. Schlachter, Milen Shishkov, Kevin Woods, Nima Tabatabaei, Tao Wu, and Guillermo J. Tearney, "Endoscopic probe optics for spectrally encoded confocal microscopy," Biomed. Opt. Express 4, 1925-1936 (2013)

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  1. G. J. Tearney, R. H. Webb, and B. E. Bouma, “Spectrally encoded confocal microscopy,” Opt. Lett.23(15), 1152–1154 (1998). [CrossRef] [PubMed]
  2. Y. K. Tao and J. A. Izatt, “Spectrally encoded confocal scanning laser ophthalmoscopy,” Opt. Lett.35(4), 574–576 (2010). [CrossRef] [PubMed]
  3. L. Golan, D. Yeheskely-Hayon, L. Minai, and D. Yelin, “High-speed interferometric spectrally encoded flow cytometry,” Opt. Lett.37(24), 5154–5156 (2012). [CrossRef] [PubMed]
  4. R. Kiesslich, L. Gossner, M. Goetz, A. Dahlmann, M. Vieth, M. Stolte, A. Hoffman, M. Jung, B. Nafe, P. R. Galle, and M. F. Neurath, “In vivo histology of Barrett’s esophagus and associated neoplasia by confocal laser endomicroscopy,” Clin. Gastroenterol. Hepatol.4(8), 979–987 (2006). [CrossRef] [PubMed]
  5. S. Kitabatake, Y. Niwa, R. Miyahara, A. Ohashi, T. Matsuura, Y. Iguchi, Y. Shimoyama, T. Nagasaka, O. Maeda, T. Ando, N. Ohmiya, A. Itoh, Y. Hirooka, and H. Goto, “Confocal endomicroscopy for the diagnosis of gastric cancer in vivo,” Endoscopy38(11), 1110–1114 (2006). [CrossRef] [PubMed]
  6. A. M. Buchner, M. W. Shahid, M. G. Heckman, M. Krishna, M. Ghabril, M. Hasan, J. E. Crook, V. Gomez, M. Raimondo, T. Woodward, H. C. Wolfsen, and M. B. Wallace, “Comparison of Probe-Based Confocal Laser Endomicroscopy With Virtual Chromoendoscopy for Classification of Colon Polyps,” Gastroenterology138(3), 834–842 (2010). [CrossRef] [PubMed]
  7. V. Becker, T. Vercauteren, C. H. von Weyhern, C. Prinz, R. M. Schmid, and A. Meining, “High-resolution miniprobe-based confocal microscopy in combination with video mosaicing (with video),” Gastrointest. Endosc.66(5), 1001–1007 (2007). [CrossRef] [PubMed]
  8. D. Yelin, C. Boudoux, B. E. Bouma, and G. J. Tearney, “Large area confocal microscopy,” Opt. Lett.32(9), 1102–1104 (2007). [CrossRef] [PubMed]
  9. D. Kang, H. Yoo, P. Jillella, B. E. Bouma, and G. J. Tearney, “Comprehensive volumetric confocal microscopy with adaptive focusing,” Biomed. Opt. Express2(6), 1412–1422 (2011). [CrossRef] [PubMed]
  10. S. C. Schlachter, D. Kang, M. J. Gora, P. Vacas-Jacques, T. Wu, R. W. Carruth, E. J. Wilsterman, B. E. Bouma, K. Woods, and G. J. Tearney, “Spectrally encoded confocal microscopy of esophageal tissues at 100 kHz line rate,” Biomed. Opt. Express4(9), 1636–1645 (2013). [CrossRef]
  11. C. Boudoux, “Wavelength swept spectrally encoded confocal microscopy for biological and clinical applications,” (MIT, Cambridge, 2007).
  12. D. Yelin, B. E. Bouma, S. H. Yun, and G. J. Tearney, “Double-clad fiber for endoscopy,” Opt. Lett.29(20), 2408–2410 (2004). [CrossRef] [PubMed]
  13. B. J. Vakoc, M. Shishko, S. H. Yun, W.-Y. Oh, M. J. Suter, A. E. Desjardins, J. A. Evans, N. S. Nishioka, G. J. Tearney, and B. E. Bouma, “Comprehensive esophageal microscopy by using optical frequency-domain imaging (with video),” Gastrointest. Endosc.65(6), 898–905 (2007). [CrossRef] [PubMed]
  14. D. Kang, M. J. Suter, C. Boudoux, H. Yoo, P. S. Yachimski, W. P. Puricelli, N. S. Nishioka, M. Mino-Kenudson, G. Y. Lauwers, B. E. Bouma, and G. J. Tearney, “Comprehensive imaging of gastroesophageal biopsy samples by spectrally encoded confocal microscopy,” Gastrointest. Endosc.71(1), 35–43 (2010). [CrossRef] [PubMed]
  15. DuPont, “DuPont FEP Film”, retrieved http://www2.dupont.com/Teflon_Industrial/en_US/assets/downloads/h55007.pdf .
  16. D. K. Kang, M. J. Suter, C. Boudoux, P. S. Yachimski, W. P. Puricelli, N. S. Nishioka, M. Mino-Kenudson, G. Y. Lauwers, B. E. Bouma, and G. J. Tearney, “Co-registered spectrally encoded confocal microscopy and optical frequency domain imaging system,” J. Microsc.239(2), 87–91 (2010). [PubMed]
  17. C. Glazowski and M. Rajadhyaksha, “Optimal detection pinhole for lowering speckle noise while maintaining adequate optical sectioning in confocal reflectance microscopes,” J. Biomed. Opt.17(8), 085001 (2012). [CrossRef] [PubMed]
  18. S. Lemire-Renaud, M. Rivard, M. Strupler, D. Morneau, F. i. Verpillat, X. Daxhelet, N. Godbout, and C. Boudoux, “Double-clad fiber coupler for endoscopy,” Opt. Express18(10), 9755–9764 (2010). [CrossRef] [PubMed]
  19. G. Ren, P. Shum, J. Hu, X. Yu, and Y. Gong, “Fabrication of all-solid photonic bandgap fiber coupler,” Opt. Lett.32(21), 3059–3061 (2007). [CrossRef] [PubMed]
  20. M. Guelrud, I. Herrera, H. Essenfeld, and J. Castro, “Enhanced magnification endoscopy: A new technique to identify specialized intestinal metaplasia in Barrett’s esophagus,” Gastrointest. Endosc.53(6), 559–565 (2001). [CrossRef] [PubMed]
  21. J. L. Vázquez-Iglesias, P. Alonso-Aguirre, M. T. Diz-Lois, M. A. Vázquez-Millán, A. Alvarez, and M. J. Lorenzo, “Acetic acid allows effective selection of areas for obtaining biopsy samples in Barrett’s esophagus,” Eur. J. Gastroenterol. Hepatol.19(3), 187–193 (2007). [CrossRef] [PubMed]

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