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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1937–1945

Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector

Keisuke Isobe, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 10, pp. 1937-1945 (2013)
http://dx.doi.org/10.1364/BOE.4.001937


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Abstract

A spatial overlap modulation (SPOM) technique is a nonlinear optical microscopy technique which enhances the three-dimensional spatial resolution and rejects the out-of-focus background limiting the imaging depth inside a highly scattering sample. Here, we report on the implementation of SPOM in which beam pointing modulation is achieved by an electro-optic deflector. The modulation and demodulation frequencies are enhanced to 200 kHz and 400 kHz, respectively, resulting in a 200-fold enhancement compared with the previously reported system. The resolution enhancement and suppression of the out-of-focus background are demonstrated by sum-frequency-generation imaging of pounded granulated sugar and deep imaging of fluorescent beads in a tissue-like phantom, respectively.

© 2013 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 11, 2013
Revised Manuscript: July 17, 2013
Manuscript Accepted: August 12, 2013
Published: September 4, 2013

Virtual Issues
Novel Techniques in Microscopy (2013) Biomedical Optics Express

Citation
Keisuke Isobe, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, and Katsumi Midorikawa, "Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector," Biomed. Opt. Express 4, 1937-1945 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-10-1937


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References

  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science248(4951), 73–76 (1990). [CrossRef] [PubMed]
  2. K. König, “Multiphoton microscopy in life sciences,” J. Microsc.200(2), 83–104 (2000). [CrossRef] [PubMed]
  3. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol.21(11), 1369–1377 (2003). [CrossRef] [PubMed]
  4. P. J. Campagnola and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol.21(11), 1356–1360 (2003). [CrossRef] [PubMed]
  5. J. Squier and M. Müller, “High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging,” Rev. Sci. Instrum.72(7), 2855–2867 (2001). [CrossRef]
  6. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, “Nonlinear scanning laser microscopy by third harmonic generation,” Appl. Phys. Lett.70(8), 922–924 (1997). [CrossRef]
  7. M. D. Duncan, J. Reintjes, and T. J. Manuccia, “Scanning coherent anti-Stokes Raman microscope,” Opt. Lett.7(8), 350–352 (1982). [CrossRef] [PubMed]
  8. A. Zumbusch, G. R. Holtom, and X. S. Xie, “Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,” Phys. Rev. Lett.82(20), 4142–4145 (1999). [CrossRef]
  9. K. Isobe, S. Kataoka, R. Murase, W. Watanabe, T. Higashi, S. Kawakami, S. Matsunaga, K. Fukui, and K. Itoh, “Stimulated parametric emission microscopy,” Opt. Express14(2), 786–793 (2006). [CrossRef] [PubMed]
  10. K. Isobe, T. Kawasumi, T. Tamaki, S. Kataoka, Y. Ozeki, and K. Itoh, “Three-dimensional profiling of refractive index distribution inside transparent materials by use of nonresonant four-wave mixing microscopy,” Appl. Phys. Express1, 022006 (2008). [CrossRef]
  11. D. Fu, T. Ye, T. E. Matthews, G. Yurtsever, and W. S. Warren, “Two-color, two-photon, and excited-state absorption microscopy,” J. Biomed. Opt.12(5), 054004 (2007). [CrossRef] [PubMed]
  12. D. Fu, T. Ye, T. E. Matthews, B. J. Chen, G. Yurtserver, and W. S. Warren, “High-resolution in vivo imaging of blood vessels without labeling,” Opt. Lett.32(18), 2641–2643 (2007). [CrossRef] [PubMed]
  13. C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, “Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy,” Science322(5909), 1857–1861 (2008). [CrossRef] [PubMed]
  14. P. Nandakumar, A. Kovalev, and A. Volkmer, “Vibrational imaging based on stimulated Raman scattering microscopy,” New J. Phys.11(3), 033026 (2009). [CrossRef]
  15. Y. Ozeki, F. Dake, S. Kajiyama, K. Fukui, and K. Itoh, “Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy,” Opt. Express17(5), 3651–3658 (2009). [CrossRef] [PubMed]
  16. P. Samineni, B. Li, J. W. Wilson, W. S. Warren, and M. C. Fischer, “Cross-phase modulation imaging,” Opt. Lett.37(5), 800–802 (2012). [CrossRef] [PubMed]
  17. J. W. Wilson, P. Samineni, W. S. Warren, and M. C. Fischer, “Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope,” Biomed. Opt. Express3(5), 854–862 (2012). [CrossRef] [PubMed]
  18. P. Theer, M. T. Hasan, and W. Denk, “Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier,” Opt. Lett.28(12), 1022–1024 (2003). [CrossRef] [PubMed]
  19. P. Theer and W. Denk, “On the fundamental imaging-depth limit in two-photon microscopy,” J. Opt. Soc. Am. A23(12), 3139–3149 (2006). [CrossRef] [PubMed]
  20. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express17(16), 13354–13364 (2009). [CrossRef] [PubMed]
  21. D. Kobat, N. G. Horton, and C. Xu, “In vivo two-photon microscopy to 1.6-mm depth in mouse cortex,” J. Biomed. Opt.16(10), 106014 (2011). [CrossRef] [PubMed]
  22. N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics7(3), 205–209 (2013). [CrossRef]
  23. G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express13(6), 2153–2159 (2005). [CrossRef] [PubMed]
  24. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express13(5), 1468–1476 (2005). [CrossRef] [PubMed]
  25. M. A. A. Neil, R. Juskaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett.22(24), 1905–1907 (1997). [CrossRef] [PubMed]
  26. N. Chen, C.-H. Wong, and C. J. R. Sheppard, “Focal modulation microscopy,” Opt. Express16(23), 18764–18769 (2008). [CrossRef] [PubMed]
  27. A. Leray and J. Mertz, “Rejection of two-photon fluorescence background in thick tissue by differential aberration imaging,” Opt. Express14(22), 10565–10573 (2006). [CrossRef] [PubMed]
  28. N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods7(2), 141–147 (2010). [CrossRef] [PubMed]
  29. Z. Chen, L. Wei, X. Zhu, and W. Min, “Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores,” Opt. Express20(17), 18525–18536 (2012). [CrossRef] [PubMed]
  30. K. Isobe, A. Suda, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process,” Biomed. Opt. Express1(3), 791–797 (2010). [CrossRef] [PubMed]
  31. K. Isobe, H. Kawano, T. Takeda, A. Suda, A. Kumagai, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy,” Biomed. Opt. Express3(7), 1594–1608 (2012).K. Isobe, H. Kawano, T. Takeda, A. Suda, A. Kumagai, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Spatial overlap modulation nonlinear optical microscopy,” in Conference on Lasers and Electro-Optics 2012, OSA Technical Digest (online) (Optical Society of America, 2012), paper JW3G.4. [CrossRef] [PubMed]
  32. J. Miyazu, T. Imai, S. Toyoda, M. Sasaura, S. Yagi, K. Kato, Y. Sasaki, and K. Fujiura, “New beam scanning model for high-speed operation using KTa1-xNbxO3 Crystals,” Appl. Phys. Express4(11), 111501 (2011). [CrossRef]
  33. S. Yagi, K. Naganuma, T. Imai, Y. Shibata, S. Ishibashi, Y. Sasaki, M. Sasaura, K. Fujiura, and K. Kato, “A mechanical-free 150-kHz repetition swept light source incorporated a KTN electro-optic deflector,” Proc. SPIE7889, 78891J, 78891J-6 (2011). [CrossRef]

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