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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1978–1990

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast

Conor Leahy, Harsha Radhakrishnan, and Vivek J. Srinivasan  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 10, pp. 1978-1990 (2013)

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We present volumetric imaging and computational techniques to quantify neuronal and myelin architecture with intrinsic scattering contrast. Using spectral / Fourier domain Optical Coherence Microscopy (OCM) and software focus-tracking we validate imaging of neuronal cytoarchitecture and demonstrate quantification in the rodent cortex in vivo. Additionally, by ex vivo imaging in conjunction with optical clearing techniques, we demonstrate that intrinsic scattering contrast is preserved in the brain, even after sacrifice and fixation. We volumetrically image cytoarchitecture and myeloarchitecture ex vivo across the entire depth of the rodent cortex. Cellular-level imaging up to the working distance of our objective (~3 mm) is demonstrated ex vivo. Architectonic features show the expected laminar characteristics; moreover, changes in contrast after the application of acetic acid suggest that entire neuronal cell bodies are responsible for the “negative contrast” present in the images. Clearing and imaging techniques that preserve tissue architectural integrity have the potential to enable non-invasive studies of the brain during development, disease, and remodeling, even in samples where exogenous labeling is impractical.

© 2013 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(290.1350) Scattering : Backscattering

ToC Category:
Optical Coherence Tomography

Original Manuscript: June 17, 2013
Revised Manuscript: August 21, 2013
Manuscript Accepted: August 22, 2013
Published: September 5, 2013

Virtual Issues
Novel Techniques in Microscopy (2013) Biomedical Optics Express

Conor Leahy, Harsha Radhakrishnan, and Vivek J. Srinivasan, "Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast," Biomed. Opt. Express 4, 1978-1990 (2013)

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