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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 1991–2002

Precise, motion-free polarization control in Second Harmonic Generation microscopy using a liquid crystal modulator in the infinity space

Chi-Hsiang Lien, Karissa Tilbury, Shean-Jen Chen, and Paul J. Campagnola  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 10, pp. 1991-2002 (2013)
http://dx.doi.org/10.1364/BOE.4.001991


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Abstract

Second Harmonic Generation (SHG) microscopy coupled with polarization analysis has great potential for use in tissue characterization, as molecular and supramolecular structural details can be extracted. Such measurements are difficult to perform quickly and accurately. Here we present a new method that uses a liquid crystal modulator (LCM) located in the infinity space of a SHG laser scanning microscope that allows the generation of any desired linear or circular polarization state. As the device contains no moving parts, polarization can be rotated accurately and faster than by manual or motorized control. The performance in terms of polarization purity was validated using Stokes vector polarimetry, and found to have minimal residual polarization ellipticity. SHG polarization imaging characteristics were validated against well-characterized specimens having cylindrical and/or linear symmetries. The LCM has a small footprint and can be implemented easily in any standard microscope and is cost effective relative to other technologies.

© 2013 OSA

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.2620) Nonlinear optics : Harmonic generation and mixing
(180.4315) Microscopy : Nonlinear microscopy
(110.5405) Imaging systems : Polarimetric imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Microscopy

History
Original Manuscript: July 5, 2013
Revised Manuscript: August 22, 2013
Manuscript Accepted: August 23, 2013
Published: September 5, 2013

Virtual Issues
Optical Molecular Probes, Imaging, and Drug Delivery (2013) Biomedical Optics Express

Citation
Chi-Hsiang Lien, Karissa Tilbury, Shean-Jen Chen, and Paul J. Campagnola, "Precise, motion-free polarization control in Second Harmonic Generation microscopy using a liquid crystal modulator in the infinity space," Biomed. Opt. Express 4, 1991-2002 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-10-1991


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