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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 2150–2165

Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography

Yu Gan and Christine P. Fleming  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 10, pp. 2150-2165 (2013)

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Abnormal changes in orientation of myofibers are associated with various cardiac diseases such as arrhythmia, irregular contraction, and cardiomyopathy. To extract fiber information, we present a method of quantifying fiber orientation and reconstructing three-dimensional tractography of myofibers using optical coherence tomography (OCT). A gradient based algorithm was developed to quantify fiber orientation in three dimensions and particle filtering technique was employed to track myofibers. Prior to image processing, three-dimensional image data set were acquired from all cardiac chambers and ventricular septum of swine hearts using OCT system without optical clearing. The algorithm was validated through rotation test and comparison with manual measurements. The experimental results demonstrate that we are able to visualize three-dimensional fiber tractography in myocardium tissues.

© 2013 Optical Society of America

OCIS Codes
(100.0100) Image processing : Image processing
(110.4500) Imaging systems : Optical coherence tomography
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Image Processing

Original Manuscript: August 2, 2013
Revised Manuscript: September 5, 2013
Manuscript Accepted: September 9, 2013
Published: September 13, 2013

Yu Gan and Christine P. Fleming, "Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography," Biomed. Opt. Express 4, 2150-2165 (2013)

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