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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 10 — Oct. 1, 2013
  • pp: 2269–2283

Low-cost laser speckle contrast imaging of blood flow using a webcam

Lisa M. Richards, S. M. Shams Kazmi, Janel L. Davis, Katherine E. Olin, and Andrew K. Dunn  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 10, pp. 2269-2283 (2013)

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Laser speckle contrast imaging has become a widely used tool for dynamic imaging of blood flow, both in animal models and in the clinic. Typically, laser speckle contrast imaging is performed using scientific-grade instrumentation. However, due to recent advances in camera technology, these expensive components may not be necessary to produce accurate images. In this paper, we demonstrate that a consumer-grade webcam can be used to visualize changes in flow, both in a microfluidic flow phantom and in vivo in a mouse model. A two-camera setup was used to simultaneously image with a high performance monochrome CCD camera and the webcam for direct comparison. The webcam was also tested with inexpensive aspheric lenses and a laser pointer for a complete low-cost, compact setup ($90, 5.6 cm length, 25 g). The CCD and webcam showed excellent agreement with the two-camera setup, and the inexpensive setup was used to image dynamic blood flow changes before and after a targeted cerebral occlusion.

© 2013 OSA

OCIS Codes
(040.1490) Detectors : Cameras
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Speckle Imaging and Diagnostics

Original Manuscript: July 10, 2013
Revised Manuscript: August 23, 2013
Manuscript Accepted: August 24, 2013
Published: September 26, 2013

Lisa M. Richards, S. M. Shams Kazmi, Janel L. Davis, Katherine E. Olin, and Andrew K. Dunn, "Low-cost laser speckle contrast imaging of blood flow using a webcam," Biomed. Opt. Express 4, 2269-2283 (2013)

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