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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2376–2382

High-speed hyperspectral Raman imaging for label-free compositional microanalysis

Ji Qi, Jingting Li, and Wei-Chuan Shih  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 11, pp. 2376-2382 (2013)
http://dx.doi.org/10.1364/BOE.4.002376


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Abstract

We present high-speed hyperspectral Raman imaging with integrated active-illumination for label-free compositional microanalysis. We show that high-quality Raman spectra can be acquired from as many as ~1,000 spots/sec semi-randomly distributed among a ~100x100 μm2 area without mechanical scanning. We demonstrate rapid data acquisition from three types of samples: 1) uniform, strong Raman scatterers, e.g., silicon substrates; 2) non-uniform, medium-strength Raman scatterers, e.g., polymer microparticles; and, 3) non-uniform, relatively weak Raman scatterers, e.g., bacterial spores. We compare the system performance to that of point-scan with an electron-multiplied CCD camera, as implemented in some commercial systems. The results suggest that our system not only provides significant imaging speed advantage for various types of samples, but also permits substantially longer integration time per spot, leading to superior signal-to-noise ratio data. Our system enables the rapid collection of high quality Raman spectra for reliable and robust compositional microanalysis that are potentially transformative in applications such as semiconductor material and device, polymer blend and biomedicine.

© 2013 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1530) Medical optics and biotechnology : Cell analysis
(180.5655) Microscopy : Raman microscopy

ToC Category:
Spectroscopic Diagnostics

History
Original Manuscript: August 2, 2013
Revised Manuscript: September 16, 2013
Manuscript Accepted: October 2, 2013
Published: October 8, 2013

Virtual Issues
Advances in Optics for Biotechnology, Medicine and Surgery (2013) Biomedical Optics Express

Citation
Ji Qi, Jingting Li, and Wei-Chuan Shih, "High-speed hyperspectral Raman imaging for label-free compositional microanalysis," Biomed. Opt. Express 4, 2376-2382 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-11-2376


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