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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2546–2554

Application of quantitative second-harmonic generation microscopy to dynamic conditions

Mohammad M. Kabir, V. V. G. Krishna Inavalli, Tung-Yuen Lau, and Kimani C. Toussaint, Jr.  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 11, pp. 2546-2554 (2013)
http://dx.doi.org/10.1364/BOE.4.002546


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Abstract

We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.

© 2013 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: July 2, 2013
Revised Manuscript: August 22, 2013
Manuscript Accepted: October 14, 2013
Published: October 21, 2013

Virtual Issues
Novel Techniques in Microscopy (2013) Biomedical Optics Express

Citation
Mohammad M. Kabir, V. V. G. Krishna Inavalli, Tung-Yuen Lau, and Kimani C. Toussaint, "Application of quantitative second-harmonic generation microscopy to dynamic conditions," Biomed. Opt. Express 4, 2546-2554 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-11-2546


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