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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 11 — Nov. 1, 2013
  • pp: 2644–2656

Re-scan confocal microscopy: scanning twice for better resolution

Giulia M.R. De Luca, Ronald M.P. Breedijk, Rick A.J. Brandt, Christiaan H.C. Zeelenberg, Babette E. de Jong, Wendy Timmermans, Leila Nahidi Azar, Ron A. Hoebe, Sjoerd Stallinga, and Erik M.M. Manders  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 11, pp. 2644-2656 (2013)
http://dx.doi.org/10.1364/BOE.4.002644


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Abstract

We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.

© 2013 Optical Society of America

OCIS Codes
(110.2990) Imaging systems : Image formation theory
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: July 30, 2013
Revised Manuscript: September 20, 2013
Manuscript Accepted: September 23, 2013
Published: October 25, 2013

Citation
Giulia M.R. De Luca, Ronald M.P. Breedijk, Rick A.J. Brandt, Christiaan H.C. Zeelenberg, Babette E. de Jong, Wendy Timmermans, Leila Nahidi Azar, Ron A. Hoebe, Sjoerd Stallinga, and Erik M.M. Manders, "Re-scan confocal microscopy: scanning twice for better resolution," Biomed. Opt. Express 4, 2644-2656 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-11-2644


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