OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 12 — Dec. 1, 2013
  • pp: 2763–2768

Characterization of ovarian tissue based on quantitative analysis of photoacoustic microscopy images

Tianheng Wang, Yi Yang, Umar Alqasemi, Patrick D. Kumavor, Xiaohong Wang, Melinda Sanders, Molly Brewer, and Quing Zhu  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 12, pp. 2763-2768 (2013)
http://dx.doi.org/10.1364/BOE.4.002763


View Full Text Article

Enhanced HTML    Acrobat PDF (1391 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In this paper, human ovarian tissue with malignant and benign features was imaged ex vivo using an optical-resolution photoacoustic microscopy (OR-PAM) system. The feasibility of PAM to differentiate malignant from normal ovarian tissues was explored by comparing the PAM images morphologically. Based on the observed differences between PAM images of normal and malignant ovarian tissues in microvasculature features and distributions, seven features were quantitatively extracted from the PAM images, and a logistic model was used to classify ovaries as normal or malignant. 106 PAM images from 18 ovaries were studied. 57 images were used to train the seven-parameter logistic model, and a specificity of 92.1% and a sensitivity of 89.5% were achieved; 49 images were then tested, and a specificity of 81.3% and a sensitivity of 88.2% were achieved. These preliminary results demonstrate the feasibility of our PAM system in mapping microvasculature networks as well as characterizing the ovarian tissue, and could be extremely valuable in assisting surgeons for in vivo evaluation of ovarian tissue during minimally invasive surgery.

© 2013 Optical Society of America

OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Optics in Cancer Research

History
Original Manuscript: August 14, 2013
Revised Manuscript: October 20, 2013
Manuscript Accepted: October 22, 2013
Published: November 6, 2013

Citation
Tianheng Wang, Yi Yang, Umar Alqasemi, Patrick D. Kumavor, Xiaohong Wang, Melinda Sanders, Molly Brewer, and Quing Zhu, "Characterization of ovarian tissue based on quantitative analysis of photoacoustic microscopy images," Biomed. Opt. Express 4, 2763-2768 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-12-2763


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. A. Brewer, U. Utzinger, J. K. Barton, J. B. Hoying, N. D. Kirkpatrick, W. R. Brands, J. R. Davis, K. Hunt, S. J. Stevens, and A. F. Gmitro, “Imaging of the ovary,” Technol. Cancer Res. Treat.3(6), 617–627 (2004). [PubMed]
  2. M. Xu and L. V. Wang, “Photoacoustic imaging in biomedicine,” Rev. Sci. Instrum.77(4), 041101 (2006). [CrossRef]
  3. L. V. Wang, “Prospects of photoacoustic tomography,” Med. Phys.35(12), 5758–5767 (2008). [CrossRef] [PubMed]
  4. A. Aguirre, P. Guo, J. Gamelin, S. Yan, M. M. Sanders, M. Brewer, and Q. Zhu, “Coregistered three-dimensional ultrasound and photoacoustic imaging system for ovarian tissue characterization,” J. Biomed. Opt.14(5), 054014 (2009). [CrossRef] [PubMed]
  5. A. Aguirre, Y. Ardeshirpour, M. M. Sanders, M. Brewer, and Q. Zhu, “Potential role of coregistered photoacoustic and ultrasound imaging in ovarian cancer detection and characterization,” Transl Oncol4(1), 29–37 (2011). [PubMed]
  6. U. Alqasemi, P. Kumavor, A. Aguirre, and Q. Zhu, “Recognition algorithm for assisting ovarian cancer diagnosis from coregistered ultrasound and photoacoustic images: ex vivo study,” J. Biomed. Opt.17(12), 126003 (2012). [CrossRef] [PubMed]
  7. P. Kumavor, U. Alqasemi, B. Tavakoli, H. Li, Y. Yang, X. Sun, E. Warych, and Q. Zhu, “Co-registered pulse-echo/photoacoustic transvaginal probe for real time imaging of ovarian tissue,” J. Biophotonics. 1-10, (2013).
  8. K. Maslov, G. Stoica, and L. V. Wang, “In vivo dark-field reflection-mode photoacoustic microscopy,” Opt. Lett.30(6), 625–627 (2005). [CrossRef] [PubMed]
  9. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol.24(7), 848–851 (2006). [CrossRef] [PubMed]
  10. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, “Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries,” Opt. Lett.33(9), 929–931 (2008). [CrossRef] [PubMed]
  11. R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, In vivo realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer,” Opt. Express16(11), 7915–7928 (2008). [CrossRef] [PubMed]
  12. L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nat. Photonics3(9), 503–509 (2009). [CrossRef] [PubMed]
  13. Z. Xie, S. Jiao, H. F. Zhang, and C. A. Puliafito, “Laser-scanning optical-resolution photoacoustic microscopy,” Opt. Lett.34(12), 1771–1773 (2009). [CrossRef] [PubMed]
  14. S. Hu, P. Yan, K. Maslov, J. M. Lee, and L. V. Wang, “Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy,” Opt. Lett.34(24), 3899–3901 (2009). [CrossRef] [PubMed]
  15. B. Rao, L. Li, K. Maslov, and L. V. Wang, “Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging,” Opt. Lett.35(10), 1521–1523 (2010). [CrossRef] [PubMed]
  16. C. Zhang, K. Maslov, and L. V. Wang, “Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo,” Opt. Lett.35(19), 3195–3197 (2010). [CrossRef] [PubMed]
  17. S. Hu, K. Maslov, and L. V. Wang, “Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed,” Opt. Lett.36(7), 1134–1136 (2011). [CrossRef] [PubMed]
  18. Z. Xie, W. Roberts, P. Carson, X. Liu, C. Tao, and X. Wang, “Evaluation of bladder microvasculature with high-resolution photoacoustic imaging,” Opt. Lett.36(24), 4815–4817 (2011). [CrossRef] [PubMed]
  19. C. Zhang, K. Maslov, S. Hu, R. Chen, Q. Zhou, K. K. Shung, and L. V. Wang, “Reflection-mode submicron-resolution in vivo photoacoustic microscopy,” J. Biomed. Opt.17(2), 020501 (2012). [CrossRef] [PubMed]
  20. Z. Guo, C. Favazza, A. Garcia-Uribe, and L. V. Wang, “Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime,” J. Biomed. Opt.17(6), 066011 (2012). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited