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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 12 — Dec. 1, 2013
  • pp: 2769–2780

Assessing the mechanical properties of tissue-mimicking phantoms at different depths as an approach to measure biomechanical gradient of crystalline lens

Shang Wang, Salavat Aglyamov, Andrei Karpiouk, Jiasong Li, Stanislav Emelianov, Fabrice Manns, and Kirill V. Larin  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 12, pp. 2769-2780 (2013)

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We demonstrate the feasibility of using the dominant frequency of the sample surface response to a mechanical stimulation as an effective indicator for sensing the depthwise distribution of elastic properties in transparent layered phantom samples simulating the cortex and nucleus of the crystalline lens. Focused ultrasound waves are used to noninvasively interrogate the sample surface. A phase-sensitive optical coherence tomography system is utilized to capture the surface dynamics over time with nanometer scale sensitivity. Spectral analysis is performed on the sample surface response to ultrasound stimulation and the dominant frequency is calculated under particular loading parameters. Pilot experiments were conducted on homogeneous and layered tissue-mimicking phantoms. Results indicate that the mechanical layers located at different depths introduce different frequencies to the sample surface response, which are correlated with the depth-dependent elasticity of the sample. The duration and the frequency of the ultrasound excitation are also investigated for their influences on this spectrum-based detection. This noninvasive method may be potentially applied for localized and rapid assessment of the depth dependence of the mechanical properties of the crystalline lens.

© 2013 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(120.7280) Instrumentation, measurement, and metrology : Vibration analysis
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.7170) Medical optics and biotechnology : Ultrasound

ToC Category:
Ophthalmology Applications

Original Manuscript: September 17, 2013
Revised Manuscript: November 5, 2013
Manuscript Accepted: November 6, 2013
Published: November 8, 2013

Shang Wang, Salavat Aglyamov, Andrei Karpiouk, Jiasong Li, Stanislav Emelianov, Fabrice Manns, and Kirill V. Larin, "Assessing the mechanical properties of tissue-mimicking phantoms at different depths as an approach to measure biomechanical gradient of crystalline lens," Biomed. Opt. Express 4, 2769-2780 (2013)

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