OSA's Digital Library

Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 2 — Feb. 1, 2013
  • pp: 187–192

Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays

Xue Zhe Li, Sookyung Kim, Wonhyung Cho, and Seung-Yop Lee  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 2, pp. 187-192 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (2493 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



In this study, we propose a new detection method of nanoparticle-enhanced human papillomavirus genotyping microarrays using a DVD optical pick-up with a photodiode. The HPV genotyping DNA chip was labeled using Au/Ag core-shell nanoparticles, prepared on a treatment glass substrate. Then, the bio information of the HPV genotyping target DNA was detected by measuring the difference of the optical signals between the DNA spots and the background parts for cervical cancer diagnosis. Moreover the approximate linear relationship between the concentration of the HPV genotyping target DNA and the optical signal depending on the density of Au/Ag core-shell nanoparticles was obtained by performing a spot finding algorithm. It is shown that the nanoparticle-labeled HPV genotyping target DNA can be measured and quantified by collecting the low-cost photodiode signal on the treatment glass chip, replacing high-cost fluorescence microarray scanners using a photomultiplier tube.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Biosensors and Molecular Diagnostics

Original Manuscript: September 10, 2012
Revised Manuscript: December 3, 2012
Manuscript Accepted: December 19, 2012
Published: December 20, 2012

Xue Zhe Li, Sookyung Kim, Wonhyung Cho, and Seung-Yop Lee, "Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays," Biomed. Opt. Express 4, 187-192 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. J. Heller, “DNA microarray technology: devices, systems, and applications,” Annu. Rev. Biomed. Eng.4(1), 129–153 (2002). [CrossRef] [PubMed]
  2. M. Schena, DNA Chip Analysis (Wiley, 2003).
  3. M. Schena, D. Shalon, R. W. Davis, and P. O. Brown, “Quantitative monitoring of gene expression patterns with a complementary DNA microarray,” Science270(5235), 467–470 (1995). [CrossRef] [PubMed]
  4. G. Hamilton, N. Brown, V. Oseroff, B. Huey, R. Segraves, D. Sudar, J. Kumler, D. Albertson, and D. Pinkel, “A large field CCD system for quantitative imaging of microarrays,” Nucleic Acids Res.34(8), e58–e72 (2006). [CrossRef] [PubMed]
  5. Y. Ardeshirpour, M. J. Deen, and S. Shirani, “Two-dimensional CMOS-based image sensor system for fluorescent detection,” Can. J. Electr. Comput. Eng.29(4), 231–235 (2004). [CrossRef]
  6. T. A. Taton, C. A. Mirkin, and R. L. Letsinger, “Scanometric DNA array detection with nanoparticle probes,” Science289(5485), 1757–1760 (2000). [CrossRef] [PubMed]
  7. N. C. Tansil and Z. Gao, “Nanoparticles in biomolecular detection,” Nano Today1(1), 28–37 (2006). [CrossRef]
  8. A. J. Haes and R. P. Van Duyne, “A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles,” J. Am. Chem. Soc.124(35), 10596–10604 (2002). [CrossRef] [PubMed]
  9. G. A. Blab, L. Cognet, S. Berciaud, I. Alexandre, D. Husar, J. Remacle, and B. Lounis, “Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement,” Biophys. J.90(1), L13–L15 (2006). [CrossRef] [PubMed]
  10. K.-H. Kim, S.-Y. Lee, S. Kim, and S. G. Jeong, “DNA microarray scanner with a DVD pick-up head,” Curr. Appl. Phys.8(6), 687–691 (2008). [CrossRef]
  11. K.-H. Kim, S.-Y. Lee, S. Kim, S.-H. Lee, and S.-G. Jeong, “A new DNA chip detection mechanism using optical pick-up actuators,” Microsyst. Technol.13(8-10), 1359–1369 (2007). [CrossRef]
  12. L. I. Segerink, M. J. Koster, A. J. Sprenkels, and A. van den Berg, “A low-cost 2D fluorescence detection system for μm sized beads on-chip,” Lab Chip12(10), 1780–1783 (2012). [CrossRef] [PubMed]
  13. S. E. McFadden and L. Schumann, “The role of human papillomavirus in screening for cervical cancer,” J. Am. Acad. Nurse Pract.13(3), 116–126, quiz 127–128 (2001). [CrossRef] [PubMed]
  14. F. X. Bosch and S. de Sanjosé, “Human papillomavirus in cervical cancer,” Curr. Oncol. Rep.4(2), 175–184 (2002). [CrossRef] [PubMed]
  15. M. B. Miller and Y. W. Tang, “Basic concepts of microarrays and potential applications in clinical microbiology,” Clin. Microbiol. Rev.22(4), 611–633 (2009). [CrossRef] [PubMed]
  16. H. J. An, N. H. Cho, S. Y. Lee, I. H. Kim, C. Lee, S. J. Kim, M. S. Mun, S. H. Kim, and J. K. Jeong, “Correlation of cervical carcinoma and precancerous lesions with human papillomavirus (HPV) genotypes detected with the HPV DNA chip microarray method,” Cancer97(7), 1672–1680 (2003). [CrossRef] [PubMed]
  17. D.-K. Lim, I.-J. Kim, and J.-M. Nam, “DNA-embedded Au/Ag core-shell nanoparticles,” Chem. Commun. (Camb.)42(42), 5312–5314 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited