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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 2 — Feb. 1, 2013
  • pp: 331–348

Combined hemoglobin and fluorescence diffuse optical tomography for breast tumor diagnosis: a pilot study on time-domain methodology

Wei Zhang, Linhui Wu, Jiao Li, Xi Yi, Xin Wang, Yiming Lu, Weiting Chen, Zhongxing Zhou, Limin Zhang, Huijuan Zhao, and Feng Gao  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 2, pp. 331-348 (2013)

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A combined time-domain fluorescence and hemoglobin diffuse optical tomography (DOT) system and the image reconstruction methods are proposed for enhancing the reliability of breast-dedicated optical measurement. The system equipped with two pulsed laser diodes at wavelengths of 780 nm and 830 nm that are specific to the peak excitation and emission of the FDA-approved ICG agent, and works with a 4-channel time-correlated single photon counting device to acquire the time-resolved distributions of the light re-emissions at 32 boundary sites of tissues in a tandem serial-to-parallel mode. The simultaneous reconstruction of the two optical (absorption and scattering) and two fluorescent (yield and lifetime) properties are achieved with the respective featured-data algorithms based on the generalized pulse spectrum technique. The performances of the methodology are experimentally assessed on breast-mimicking phantoms for hemoglobin- and fluorescence-DOT alone, as well as for fluorescence-guided hemoglobin-DOT. The results demonstrate the efficacy of improving the accuracy of hemoglobin-DOT based on a priori fluorescence localization.

© 2013 OSA

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Diffuse Optical Imaging

Original Manuscript: December 21, 2012
Revised Manuscript: January 21, 2013
Manuscript Accepted: January 23, 2013
Published: January 25, 2013

Wei Zhang, Linhui Wu, Jiao Li, Xi Yi, Xin Wang, Yiming Lu, Weiting Chen, Zhongxing Zhou, Limin Zhang, Huijuan Zhao, and Feng Gao, "Combined hemoglobin and fluorescence diffuse optical tomography for breast tumor diagnosis: a pilot study on time-domain methodology," Biomed. Opt. Express 4, 331-348 (2013)

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