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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 548–558

Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas

Bruno Montcel, Laurent Mahieu-Williame, Xavier Armoiry, David Meyronet, and Jacques Guyotat  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 4, pp. 548-558 (2013)

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5-ALA-induced protoporphyrin IX (PpIX) fluorescence enables to guiding in intra-operative surgical glioma resection. However at present, it has yet to be shown that this method is able to identify infiltrative component of glioma. In extracted tumor tissues we measured a two-peaked emission in low grade gliomas and in the infiltrative component of glioblastomas due to multiple photochemical states of PpIX. The second emission peak appearing at 620 nm (shifted by 14 nm from the main peak at 634 nm) limits the sensibility of current methods to measured PpIX concentration. We propose new measured parameters, by taking into consideration the two-peaked emission, to overcome these limitations in sensitivity. These parameters clearly distinguish the solid component of glioblastomas from low grade gliomas and infiltrative component of glioblastomas.

© 2013 OSA

OCIS Codes
(000.1430) General : Biology and medicine
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Optics in Cancer Research

Original Manuscript: January 2, 2013
Revised Manuscript: February 28, 2013
Manuscript Accepted: March 8, 2013
Published: March 13, 2013

Bruno Montcel, Laurent Mahieu-Williame, Xavier Armoiry, David Meyronet, and Jacques Guyotat, "Two-peaked 5-ALA-induced PpIX fluorescence emission spectrum distinguishes glioblastomas from low grade gliomas and infiltrative component of glioblastomas," Biomed. Opt. Express 4, 548-558 (2013)

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