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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 559–568

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

Lyubov V. Titova, Ayesheshim K. Ayesheshim, Andrey Golubov, Dawson Fogen, Rocio Rodriguez-Juarez, Frank A. Hegmann, and Olga Kovalchuk  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 4, pp. 559-568 (2013)
http://dx.doi.org/10.1364/BOE.4.000559


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Abstract

Recent emergence and growing use of terahertz (THz) radiation for medical imaging and public security screening raise questions on reasonable levels of exposure and health consequences of this form of electromagnetic radiation. In particular, picosecond-duration THz pulses have shown promise for novel diagnostic imaging techniques. However, the effects of THz pulses on human cells and tissues thus far remain largely unknown. We report on the investigation of the biological effects of pulsed THz radiation on artificial human skin tissues. We observe that exposure to intense THz pulses for ten minutes leads to a significant induction of H2AX phosphorylation, indicating that THz pulse irradiation may cause DNA damage in exposed skin tissue. At the same time, we find a THz-pulse-induced increase in the levels of several proteins responsible for cell-cycle regulation and tumor suppression, suggesting that DNA damage repair mechanisms are quickly activated. Furthermore, we find that the cellular response to pulsed THz radiation is significantly different from that induced by exposure to UVA (400 nm).

© 2013 OSA

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.6930) Medical optics and biotechnology : Tissue
(170.7160) Medical optics and biotechnology : Ultrafast technology

ToC Category:
Optical Therapies and Photomodificaton

History
Original Manuscript: December 19, 2012
Revised Manuscript: February 1, 2013
Manuscript Accepted: February 14, 2013
Published: March 14, 2013

Citation
Lyubov V. Titova, Ayesheshim K. Ayesheshim, Andrey Golubov, Dawson Fogen, Rocio Rodriguez-Juarez, Frank A. Hegmann, and Olga Kovalchuk, "Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue," Biomed. Opt. Express 4, 559-568 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-4-559


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