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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 4 — Apr. 1, 2013
  • pp: 596–613

Investigation of depth-resolved nanoscale structural changes in regulated cell proliferation and chromatin decondensation

Shikhar Uttam, Rajan K. Bista, Kevin Staton, Sergey Alexandrov, Serah Choi, Christopher J. Bakkenist, Douglas J. Hartman, Randall E. Brand, and Yang Liu  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 4, pp. 596-613 (2013)
http://dx.doi.org/10.1364/BOE.4.000596


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Abstract

We present depth-resolved spatial-domain low-coherence quantitative phase microscopy, a simple approach that utilizes coherence gating to construct a depth-resolved structural feature vector quantifying sub-resolution axial structural changes at different optical depths within the sample. We show that this feature vector is independent of sample thickness variation, and identifies nanoscale structural changes in clinically prepared samples. We present numerical simulations and experimental validation to demonstrate the feasibility of the approach. We also perform experiments using unstained cells to investigate the nanoscale structural changes in regulated cell proliferation through cell cycle and chromatin decondensation induced by histone acetylation.

© 2013 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.4730) Medical optics and biotechnology : Optical pathology
(180.3170) Microscopy : Interference microscopy
(300.6300) Spectroscopy : Spectroscopy, Fourier transforms

ToC Category:
Cell Studies

History
Original Manuscript: January 9, 2013
Revised Manuscript: February 21, 2013
Manuscript Accepted: March 11, 2013
Published: March 22, 2013

Citation
Shikhar Uttam, Rajan K. Bista, Kevin Staton, Sergey Alexandrov, Serah Choi, Christopher J. Bakkenist, Douglas J. Hartman, Randall E. Brand, and Yang Liu, "Investigation of depth-resolved nanoscale structural changes in regulated cell proliferation and chromatin decondensation," Biomed. Opt. Express 4, 596-613 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-4-596


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