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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 5 — May. 1, 2013
  • pp: 652–658

Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope

David M. Huland, Kriti Charan, Dimitre G. Ouzounov, Jason S. Jones, Nozomi Nishimura, and Chris Xu  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 5, pp. 652-658 (2013)
http://dx.doi.org/10.1364/BOE.4.000652


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Abstract

We present a compact and portable three-photon gradient index (GRIN) lens endoscope system suitable for imaging of unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The lateral and axial resolution in water is 1.0 μm and 9.5 μm, respectively. The ~200 μm diameter field of view is imaged at 2 frames/s using a fiber-based excitation source at 1040 nm. Ex vivo imaging is demonstrated with unstained mouse lung at 5.9 mW average power. These results demonstrate the feasibility of three-photon GRIN lens endoscopy for optical biopsy.

© 2013 OSA

OCIS Codes
(110.2760) Imaging systems : Gradient-index lenses
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Endoscopes, Catheters and Micro-Optics

History
Original Manuscript: January 15, 2013
Revised Manuscript: March 7, 2013
Manuscript Accepted: March 15, 2013
Published: April 1, 2013

Citation
David M. Huland, Kriti Charan, Dimitre G. Ouzounov, Jason S. Jones, Nozomi Nishimura, and Chris Xu, "Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope," Biomed. Opt. Express 4, 652-658 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-5-652


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