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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 6 — Jun. 1, 2013
  • pp: 885–899

Simple buffers for 3D STORM microscopy

Nicolas Olivier, Debora Keller, Vinoth Sundar Rajan, Pierre Gönczy, and Suliana Manley  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 6, pp. 885-899 (2013)

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3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30–50 nm in the axial direction. However, there is one important requirement to perform this type of imaging: making dye molecules blink. This usually relies on the utilization of complex buffers, containing different chemicals and sensitive enzymatic systems, limiting the reproducibility of the method. We report here that the commercial mounting medium Vectashield can be used for STORM of Alexa-647, and yields images comparable or superior to those obtained with more complex buffers, especially for 3D imaging. We expect that this advance will promote the versatile utilization of 3D STORM by removing one of its entry barriers, as well as provide a more reproducible way to compare optical setups and data processing algorithms.

OCIS Codes
(100.6640) Image processing : Superresolution
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: March 19, 2013
Revised Manuscript: April 26, 2013
Manuscript Accepted: April 28, 2013
Published: May 14, 2013

Nicolas Olivier, Debora Keller, Vinoth Sundar Rajan, Pierre Gönczy, and Suliana Manley, "Simple buffers for 3D STORM microscopy," Biomed. Opt. Express 4, 885-899 (2013)

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