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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 7 — Jul. 1, 2013
  • pp: 1006–1013

Analysis of spatial lamellar distribution from adaptive-optics second harmonic generation corneal images

Juan M. Bueno, Raquel Palacios, Mary K. Chessey, and Harilaos Ginis  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 7, pp. 1006-1013 (2013)
http://dx.doi.org/10.1364/BOE.4.001006


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Abstract

The spatial organization of stromal collagen of ex-vivo corneas has been quantified in adaptive-optics second harmonic generation (SHG) images by means of an optimized Fourier transform (FT) based analysis. At a particular depth location, adjacent lamellae often present similar orientations and run parallel to the corneal surface. However this pattern might be combined with interweaved collagen bundles leading to crosshatched structures with different orientations. The procedure here reported provides us with both principal and crosshatched angles. This is also able to automatically distinguish a random distribution from a cross-shaped one, since it uses the ratio of the axes lengths of the best-fitted ellipse of the FT data as an auxiliary parameter. The technique has successfully been applied to SHG images of healthy corneas (both stroma and Bowman’s layer) of different species and to corneas undergoing cross-linking treatment.

© 2013 OSA

OCIS Codes
(110.2960) Imaging systems : Image analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Image Processing

History
Original Manuscript: March 12, 2013
Revised Manuscript: April 24, 2013
Manuscript Accepted: April 24, 2013
Published: June 4, 2013

Citation
Juan M. Bueno, Raquel Palacios, Mary K. Chessey, and Harilaos Ginis, "Analysis of spatial lamellar distribution from adaptive-optics second harmonic generation corneal images," Biomed. Opt. Express 4, 1006-1013 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-7-1006


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