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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 7 — Jul. 1, 2013
  • pp: 1061–1073

Three-dimensional mapping of oxygen tension in cortical arterioles before and after occlusion

S. M. Shams Kazmi, Anthony J. Salvaggio, Arnold D. Estrada, Michael A. Hemati, Nazariy K. Shaydyuk, Emannuel Roussakis, Theresa A. Jones, Sergei A. Vinogradov, and Andrew K. Dunn  »View Author Affiliations


Biomedical Optics Express, Vol. 4, Issue 7, pp. 1061-1073 (2013)
http://dx.doi.org/10.1364/BOE.4.001061


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Abstract

Occlusions in single cortical microvessels lead to a reduction in oxygen supply, but this decrement has not been able to be quantified in three dimensions at the level of individual vessels using a single instrument. We demonstrate a combined optical system using two-photon phosphorescence lifetime and fluorescence microscopy (2PLM) to characterize the partial pressure of oxygen (pO2) in single descending cortical arterioles in the mouse brain before and after generating a targeted photothrombotic occlusion. Integrated real-time Laser Speckle Contrast Imaging (LSCI) provides wide-field perfusion maps that are used to monitor and guide the occlusion process while 2PLM maps changes in intravascular oxygen tension. We present the technique’s utility in highlighting the effects of vascular networking on the residual intravascular oxygen tensions measured after occlusion in three dimensions.

© 2013 OSA

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.5380) Medical optics and biotechnology : Physiology
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Functional Imaging

History
Original Manuscript: May 2, 2013
Revised Manuscript: June 7, 2013
Manuscript Accepted: June 7, 2013
Published: June 10, 2013

Virtual Issues
July 16, 2013 Spotlight on Optics

Citation
S. M. Shams Kazmi, Anthony J. Salvaggio, Arnold D. Estrada, Michael A. Hemati, Nazariy K. Shaydyuk, Emannuel Roussakis, Theresa A. Jones, Sergei A. Vinogradov, and Andrew K. Dunn, "Three-dimensional mapping of oxygen tension in cortical arterioles before and after occlusion," Biomed. Opt. Express 4, 1061-1073 (2013)
http://www.opticsinfobase.org/boe/abstract.cfm?URI=boe-4-7-1061


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