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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1285–1293

In vivo two-photon imaging of the mouse retina

Robin Sharma, Lu Yin, Ying Geng, William H. Merigan, Grazyna Palczewska, Krzysztof Palczewski, David R. Williams, and Jennifer J. Hunter  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 8, pp. 1285-1293 (2013)

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Though in vivo two-photon imaging has been demonstrated in non-human primates, improvements in the signal-to-noise ratio (SNR) would greatly improve its scientific utility. In this study, extrinsic fluorophores, expressed in otherwise transparent retinal ganglion cells, were imaged in the living mouse eye using a two-photon fluorescence adaptive optics scanning laser ophthalmoscope. We recorded two orders of magnitude greater signal levels from extrinsically labeled cells relative to previous work done in two-photon autofluorescence imaging of primates. Features as small as single dendrites in various layers of the retina could be resolved and predictions are made about the feasibility of measuring functional response from cells. In the future, two-photon imaging in the intact eye may allow us to monitor the function of retinal cell classes with infrared light that minimally excites the visual response.

© 2013 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Ophthalmology Applications

Original Manuscript: March 4, 2013
Revised Manuscript: May 6, 2013
Manuscript Accepted: June 17, 2013
Published: July 9, 2013

Virtual Issues
August 13, 2013 Spotlight on Optics

Robin Sharma, Lu Yin, Ying Geng, William H. Merigan, Grazyna Palczewska, Krzysztof Palczewski, David R. Williams, and Jennifer J. Hunter, "In vivo two-photon imaging of the mouse retina," Biomed. Opt. Express 4, 1285-1293 (2013)

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