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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1318–1331

Influence of sampling window size and orientation on parafoveal cone packing density

Marco Lombardo, Sebastiano Serrao, Pietro Ducoli, and Giuseppe Lombardo  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 8, pp. 1318-1331 (2013)

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We assessed the agreement between sampling windows of different size and orientation on packing density estimates in images of the parafoveal cone mosaic acquired using a flood-illumination adaptive optics retinal camera. Horizontal and vertical oriented sampling windows of different size (320x160 µm, 160x80 µm and 80x40 µm) were selected in two retinal locations along the horizontal meridian in one eye of ten subjects. At each location, cone density tended to decline with decreasing sampling area. Although the differences in cone density estimates were not statistically significant, Bland-Altman plots showed that the agreement between cone density estimated within the different sampling window conditions was moderate. The percentage of the preferred packing arrangements of cones by Voronoi tiles was slightly affected by window size and orientation. The results illustrated the high importance of specifying the size and orientation of the sampling window used to derive cone metric estimates to facilitate comparison of different studies.

© 2013 OSA

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(110.3925) Imaging systems : Metrics
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Active and Adaptive Optics

Original Manuscript: March 7, 2013
Revised Manuscript: June 12, 2013
Manuscript Accepted: June 12, 2013
Published: July 12, 2013

Marco Lombardo, Sebastiano Serrao, Pietro Ducoli, and Giuseppe Lombardo, "Influence of sampling window size and orientation on parafoveal cone packing density," Biomed. Opt. Express 4, 1318-1331 (2013)

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