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Biomedical Optics Express

Biomedical Optics Express

  • Editor: Joseph A. Izatt
  • Vol. 4, Iss. 8 — Aug. 1, 2013
  • pp: 1332–1341

Real-time, continuous, fluorescence sensing in a freely-moving subject with an implanted hybrid VCSEL/CMOS biosensor

Thomas D. O’Sullivan, Roxana T. Heitz, Natesh Parashurama, David B. Barkin, Bruce A. Wooley, Sanjiv S. Gambhir, James S. Harris, and Ofer Levi  »View Author Affiliations

Biomedical Optics Express, Vol. 4, Issue 8, pp. 1332-1341 (2013)

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Performance improvements in instrumentation for optical imaging have contributed greatly to molecular imaging in living subjects. In order to advance molecular imaging in freely moving, untethered subjects, we designed a miniature vertical-cavity surface-emitting laser (VCSEL)-based biosensor measuring 1cm3 and weighing 0.7g that accurately detects both fluorophore and tumor-targeted molecular probes in small animals. We integrated a critical enabling component, a complementary metal-oxide semiconductor (CMOS) read-out integrated circuit, which digitized the fluorescence signal to achieve autofluorescence-limited sensitivity. After surgical implantation of the lightweight sensor for two weeks, we obtained continuous and dynamic fluorophore measurements while the subject was un-anesthetized and mobile. The technology demonstrated here represents a critical step in the path toward untethered optical sensing using an integrated optoelectronic implant.

© 2013 OSA

OCIS Codes
(130.5990) Integrated optics : Semiconductors
(130.6010) Integrated optics : Sensors
(140.2020) Lasers and laser optics : Diode lasers
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(230.5160) Optical devices : Photodetectors

ToC Category:
Small Animal Imaging and Veterinary Studies

Original Manuscript: March 7, 2013
Revised Manuscript: June 5, 2013
Manuscript Accepted: June 26, 2013
Published: July 15, 2013

Thomas D. O’Sullivan, Roxana T. Heitz, Natesh Parashurama, David B. Barkin, Bruce A. Wooley, Sanjiv S. Gambhir, James S. Harris, and Ofer Levi, "Real-time, continuous, fluorescence sensing in a freely-moving subject with an implanted hybrid VCSEL/CMOS biosensor," Biomed. Opt. Express 4, 1332-1341 (2013)

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